CellVue® Burgundy Fluorescent Cell Labeling Kit

CellVue Burgundy Fluorescent Cell Labeling Kit from LI-COR.

The CellVue Burgundy cell labeling kit uses proprietary membrane labeling technology to stably incorporate CellVue Burgundy, a fluorescent dye with long aliphatic tails, into lipid regions of the cell membrane1. The labeling buffer provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The labeling efficiency of staining is dependent upon the cell type being labeled and the membranes of the cells2, 3.

Use NIR Fluorescent Labeling for Cell Tracking and Cell Proliferation Studies

CellVue products have been reported to be useful for short-term in vitro cell proliferation studies4, cell tracking in isolated organ preparation applications5, and cell tracking using the Pearl® Small Animal Imaging System or Odyssey® CLx or Odyssey Classic Infrared Imaging System6.

Kit Components

  • CellVue Burgundy dye stock (1 vial containing 0.1 mL, 1 x 10-3 M in ethanol)
  • Diluent C (1 vial containing 10 mL, sufficient for 5 labeling reactions)

Excitation and Emission Properties of CellVue Burgundy Dye

The excitation and emission properties of CellVue Burgundy dye are compatible with a range of commercially available near-infrared fluorescent plate readers, flow cytometers, in vitro and in vivo fluorescent imaging systems, and confocal microscopes.

CellVue Burgundy Excitation and Emission Spectra
CellVue Burgundy Excitation and Emission Spectra (ex max = 683 nm; em max = 707 nm).

Data Example using CellVue Burgundy Kit

CellVue Burgundy and CellVue NIR815 stains
Two-color imaging of CellVue Burgundy and CellVue NIR815 stains with the Odyssey Imager. A monocyte cell line (U937) was labeled with CellVue Burgundy (red), and a T-cell line (SUPT1) was labeled with CellVue NIR815 (green). The standard labeling protocol was used (1 x 107 cells/mL, 2 μM dye for 2-5 min at 25 °C). Labeled cells were spotted on a glass surface (106 cells/spot), with the cell spots partially overlapping. Cell spots were imaged with the Odyssey Infrared Imager, in two distinct fluorescent channels. A. Monocytes labeled with CellVue Burgundy were detected in the 700-nm channel (red). B. T-cells labeled with CellVue NIR815 were detected in the 800-nm channel (green). C. Simultaneous imaging in both fluorescent channels clearly shows both cell populations (overlapping signals are shown in yellow). Signal bleed-through between the two channels was not observed. Images courtesy of S. van de Waarsenburg and D. Anderson, Burnet Institute, Melbourne, Australia.

References

  1. Horan, P. K., and Slezak, S. E., Nature, 340, 167-168 (1989).
  2. Horan, P. K., et al., Methods Cell Biol., 33, 469-490 (1990).
  3. Poon, R.Y., et al. In Living Color: Flow Cytometry and Cell Sorting Protocols, Diamond, R. A., and DeMaggio, S. (Eds.). p. 302-352 (Springer-Verlag, New York, 2000).
  4. Stewart, C. C., Woodring, M. L., Podniesinski, E. and Gray, B. D. Flow Cytometer in the Infrared: Inexpensive Modifications to a Commercial Instrument. Cytometry, Part A, 67A, #2, 104-111 (2005).
  5. Al-Mehdi, A-B, et al. Increased Depth of Cellular Imaging in the Intact Lung Using Far Red and Near Infrared Fluorescent Probes. Int. J. Biomed. Imag., Vol 2006, Article ID 37470, p 1-7.
  6. Thomas, D. L., et al., Experimental Manipulations of Afferent Immune Responses Influence Efferent Immune Responses to Brain Tumors. Cancer Immunol. Immunother. Sep, 57(9): 1323-33 (2008).

CellVue is a registered trademark of PTI Research, Inc, used under license. CellVue products are sold under license from PTI Research, Inc.. US Patent # 8,029,767B2.

Selected P/N: 929-90010

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