IRDye® 680LT Goat anti-Mouse IgM (µ chain specific) Secondary Antibody

IRDye 680LT Goat anti-Mouse IgM (mu chain) Secondary Antibody

Immunogen

Mouse IgM paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 - 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye® 680LT Goat anti-Mouse IgM (u chain specific) Secondary Antibody data
Western blot detection of guinea pig IgG with IRDye 680LT Donkey anti-Guinea Pig. Serial dilutions of guinea pig IgG were spiked into C32 lysate (Santa Cruz P/N sc-2205). Samples were resolved on a 10% Bis-Tris gel and transferred to Odyssey® Nitrocellulose membrane (P/N 926-31092). The membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) followed by detection with IRDye 680LT Donkey anti-Guinea Pig (P/N 926-68030).

Selected P/N: 926-68080,925-68080

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IRDye 680LT secondary antibodies are not intended for use in In-Cell Western assays or in vivo imaging applications.

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