IRDye® 800CW Azide Infrared Dye

IRDye 800CW Azide Infrared Dye for Biomolecule Labeling.

IRDye 800CW Azide provides the functionality for preferential labeling of molecules that contain either the alkyne or dibenzocyclooctyne (DBCO) group.

Learn more about Near-Infrared Fluorescent Click Chemistry Reagents.

Dye Structure

Properties

  • Chemical Formula: C54H67N6Na3O17S4
  • Molecular Weight: 1269.36 g/mol
  • Exact Mass: 1268.31
  • Correction factor at 260 – 280 nm: 3%

Absorption and Emission Spectra

Solvent Absorption Maxima Emission Maxima
Methanol 778 nm 794 nm
1X PBS 774 nm 789 nm
1:1 Methanol to 1X PBS 778 nm

Absorbance and Emission Spectra in 1X PBS

Molar Absorptivity

Solvent Molar Absorptivity (L mol-1 cm-1)
Methanol 300,000
1X PBS 240,000
1:1 Methanol to 1X PBS 270,000

Example Data

Palmitoylation of Lck kinase analyzed with a Click Chemistry IRDye reagent.​​ Lck was immunoprecipitated from metabolically-labeled Jurkat cell lysates. Palmitoylation was detected with CuAAC click chemistry and IRDye 800CW Azide Infrared Dye​ (green signals; P/N 929-60000). Total Lck was detected with anti-Lck D88 antibody and IRDye 680RD Donkey anti-Rabbit IgG​ (red; P/N 926-68073). Yellow indicates overlap of signals. The mobility shift caused by palmitoylation is observed in lanes 3-5 and 7-9 (green bands migrating more slowly than red bands). Data and image supplied by a LI-COR customer.

Selected P/N: 929-60000,929-65000,929-75050

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