IRDye® 800CW Goat anti-Mouse IgG1-Specific Secondary Antibody

IRDye 800CW Goat anti-Mouse IgG1 Secondary Antibody

Immunogen

Mouse IgG1 paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG1 covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG1. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG2a, IgG2b, IgG3, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western assay applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.

Applications

Highly recommended for:

  • Western Blot
  • In-Cell Western Assay
  • On-Cell Western Assay
  • Protein Array
  • Immunohistochemistry
  • Small Animal Imaging
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging
  • Virus Titration Assay

Formulation

IRDye 800CW secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey® Western blot detection 1:15,000 1:5,000 - 1:25,000
In-Cell Western assay detection 1:800 1:200 - 1:1,200
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye® 800CW Goat anti-Mouse IgG1 Specific Secondary Antibody data
Purified mouse subclasses were detected by Western blot with IRDye 800CW Goat anti-Mouse IgG1 (P/N 926-32350) to demonstrate that it detects the heavy chains of mouse IgG and mouse IgG1.

Selected P/N: 926-32350

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