Mouse IgM paraproteins
Purity and Specificity
Isolation of specific antibodies was accomplished by affinity chromatography using mouse IgM covalently linked to agarose. Based on ELISA and/or flow cytometry, this antibody reacts with the heavy chain of mouse IgM. This antibody was tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgG1, IgG2a, IgG3, and IgA, pooled human sera and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot applications.
Highly recommended for:
- Western Blot
- In-Cell Western™ Assay
- On-Cell Western Assay
- Protein Array
- Small Animal Imaging
- 2D Gel Detection
- Tissue Section Imaging
IRDye 680RD secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
|Odyssey® Western blot detection||1:15,000||1:5,000 - 1:25,000|
Optimum dilutions will vary and should be determined empirically.