IRDye® 680LT Donkey anti-Chicken Secondary Antibody

IRDye 680LT Donkey anti-Chicken Secondary Antibody

Immunogen

Chicken IgY, whole molecule. IgY is the original designation for the IgG-like protein found in both serum and egg yolk.

Purity and Specificity

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 - 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye® 680LT Donkey anti-Chicken Secondary Antibody data
Western blot analysis of PTEN expression in mouse PTEN transfected 293T whole cell lysate (lane 2) and non-transfected 293T lysate (lane 3). Both lysates were loaded with 2 µg total protein per lane. Odyssey® One-Color Molecular Weight Marker (P/N 928-40000) was loaded in lane 1. The blot was probed with mouse ant-PTEN (IgG2b) (Santa Cruz P/N sc-133197) and chicken anti-GAPDH (ProSci P/N XW-7214) followed by detection with IRDye 800CW Donkey anti-Mouse (P/N 926-32212) and IRDye 680LT Donkey anti-Chicken (P/N 926-68028).

Selected P/N: 926-68028,925-68028

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IRDye 680LT secondary antibodies are not intended for use in In-Cell Western assays or in vivo imaging applications.

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