IRDye 680LT Goat anti-Rat IgG Secondary Antibody

IRDye® 680LT Goat anti-Rat IgG Secondary Antibody

IRDye 680LT Goat anti-Rat IgG Secondary Antibody


Rat IgG, whole molecule

Purity and Specificity

The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, this antibody reacts with whole molecule rat IgG, and with the light chains of other rat immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse, bovine, horse, human, and rabbit serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.


Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay


IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 - 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.


  • P/N 925-68029: RRID AB_2814909
  • P/N 926-68029: RRID AB_10715073

Example Data

IRDye® 680LT Goat anti-Rat IgG Secondary Antibody data
Western blot detection of tubulin in C32 lysate. Samples loaded in 4X Protein Sample Loading Buffer (P/N 928-40004), resolved on a 10% Bis-Tris gel, and transferred to Odyssey® nitrocellulose (P/N 926-31090). The membrane was blocked with Odyssey Blocking Buffer (P/N 927-40000) and probed with rat anti-tubulin (Novus Biologicals P/N NB600-506) followed by detection with IRDye 680LT Goat anti-Rat IgG (P/N 926-68029).

Selected P/N: 926-68029,925-68029



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IRDye 680LT secondary antibodies are not intended for use in In-Cell Western assays or in vivo imaging applications.


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