IRDye® 750 NHS Ester

IRDye 750 NHS Ester to Label Lysine Residues.

IRDye 750 NHS Ester is an infrared dye with detection near 750 nm. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

This dye is not suitable for use with LI-COR Odyssey® Imaging Systems.

IRDye® 800CW NHS Ester reaction
NHS Ester reacts with a primary aliphatic amine, such as lysine.
IRDye® 800CW NHS Ester amide bond
Dye label is attached through amide bond.
IRDye Infrared Dyes Overview

Dye Structure

IRDye 750 NHS Ester structure

Properties

  • Chemical Formula: C53H60N3Na3O16S4
  • Molecular Weight: 1192.28 g/mol
  • Exact Mass: 1191.25

Absorption and Emission Spectra

Solvent Ext. Coeff. (M-1cm-1) Absorption Maxima Emission Maxima
Methanol 330,000 766 nm 776 nm
1X PBS 260,000 756 nm 776 nm
PBS: Methanol 260,000

Absorption and Emission Spectra in Methanol

IRDye 750 Absorption and Emission Spectra

To discuss purchase quantities over 1 gram or commercial development rights using IRDye infrared dyes, please contact Business Development.

Selected P/N: 929-70040,929-70041

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