IRDye® 680LT Donkey anti-Mouse IgG Secondary Antibody

IRDye 680LT Donkey anti-Mouse IgG Secondary Antibody

Immunogen

Mouse IgG

Purity and Specificity

The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with the heavy and light chains of mouse IgG, and with the light chains of mouse IgM and IgA. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, rabbit, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.

Applications

Recommended for:

  • Western Blot
  • Protein Array
  • Immunohistochemistry
  • Microscopy
  • 2D Gel Detection
  • Tissue Section Imaging

Not Recommended for:

  • Small Animal Imaging
  • In-Cell Western Assay
  • On-Cell Western Assay

Formulation

IRDye 680LT secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Odyssey Western blot detection 1:20,000 1:20,000 - 1:50,000
Other User optimized

Optimum dilutions will vary and should be determined empirically.

Example Data

IRDye® 680LT Donkey anti-Mouse IgG Secondary Antibody data
Western blot detection of actin in C32 lysate. Samples loaded in 4X Protein Sample Loading Buffer (P/N 928-40004), resolved on a 10% Bis-Tris gel, and transferred to Odyssey® nitrocellulose (P/N 926-31090). The membrane was probed with mouse anti-actin (Neomarkers P/N MS-1295) followed by detection with IRDye 680LT Donkey anti-Mouse IgG (P/N 926-68022).

Selected P/N: 926-68022,925-68022

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IRDye 680LT secondary antibodies are not intended for use in In-Cell Western assays or in vivo imaging applications.

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