Odyssey® CLx
Imaging System

Get clearer answers with accurate, reproducible Western blots and many other assays.

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Odyssey CLx
do more clx

Do more with digital fluorescence

Get consistent, reproducible digital images, without the hassles and unpredictability of film – so you can analyze your data right away and plan your next experiment.

in-cell western clx

In-Cell Western Assay

target western odyssey clx

NIR Fluorescent Western Blot

emsa gel shift odyssey clx

EMSA / Gel Shift Assay

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Tissue Section

Image courtesy of C. Kearn,
University of Washington

Go where your experiments take you

Western blots, cell-based assays, protein arrays, gel shift assays, tissue section imaging, and more are at your fingertips with near-infrared (NIR) fluorescence on the Odyssey CLx.

“We are finding that we can use the Odyssey CLx for just about everything protein-related we do in the lab.”

Dr. Jeremy Chambers, Florida International University
 

Image and analyze your samples quickly

Analyze your data right away – it’s already in digital format. One digital file contains all your image data. Record-keeping is easy and multiple exposures are a thing of the past.

To image and analyze many samples efficiently, use the Odyssey CLx to scan up to 9 mini-blots, 6 microplates, or 30 slides at the same time. The large scanning bed provides high throughput for many assays.

See the full story in a single image

See both strong and faint bands clearly in a single image acquired on the Odyssey CLx, with great sensitivity and no image saturation. Multiplex with two fluorescent colors to see even more data.

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One digital image file contains all your data so you can see both strong and faint bands clearly in a single image, without image saturation, “blowout”, or sacrificing sensitivity. Increase the consistency and reproducibility of your results by capturing a single image with the Odyssey CLx, rather than comparing multiple exposures captured under variable conditions.

“The superior dynamic range and sensitivity allows me to confidently report my data.”

Lars Engstrom, Mirati Therapeutics, SelectScience® Review
Odyssey Clx Western Blot Image Sequence 1 Odyssey Clx Western Blot Image Sequence 2 Odyssey Clx Western Blot Image Sequence 3 Odyssey Clx Western Blot Image Sequence 4 Odyssey Clx Western Blot Image Sequence 5 Odyssey Clx Western Blot Image Sequence 6 Odyssey Clx Western Blot Image Sequence 7 Odyssey Clx Western Blot Image Sequence 8 Odyssey Clx Western Blot Image Sequence 9 Odyssey Clx Western Blot Image Sequence 10 Odyssey Clx Western Blot Image Sequence 11 Odyssey Clx Western Blot Image Sequence 12 Odyssey Clx Western Blot Image Sequence 13 Odyssey Clx Western Blot Image Sequence 14 Odyssey Clx Western Blot Image Sequence 15 Odyssey Clx Western Blot Image Sequence 16 Odyssey Clx Western Blot Image Sequence 17 Odyssey Clx Western Blot Image Sequence 18 Odyssey Clx Western Blot Image Sequence 19

One image file contains the full range of your data, and changing image display settings never alters your raw data or signal intensities.

low background clx

Get better sensitivity with low image background

Powerful, precise laser excitation and specialized optics enable the Odyssey CLx to provide high signal-to-noise ratios and outstanding image quality.

High signal-to-noise ratios give you more confidence in your data and can help you reliably detect subtle changes between samples. Low background fluorescence at NIR wavelengths means high sensitivity for your blots.

Visible region (Vy3) High autofluorescence Near-infrared region (700nm) Low autofluorescence

NIR fluorescence imaging gives you low membrane fluorescence and high sensitivity. Other imagers use LEDs or diffuse white light sources that provide weak, non-specific excitation light and limit sensitivity. Visible fluorescent dyes like Cy3 have poor sensitivity, because membrane autofluorescence is so strong. Blots show detection of phospho-ERK with NIR fluorescence (700 nm) and with ECL Plex reagents.

“The brilliant signal-to-noise ratio in combination with the ability to truly quantify the data is really outstanding.”

Geir Bjørkøy, University College of Sør-Trøndelag
sensitivity accuracy clx

Enhance the accuracy of your data. The wide dynamic range of Odyssey imagers enables high-sensitivity detection without instrument saturation.

See your bands reliably with stable fluorescent signals

Fluorescent signals are stable and unaffected by timing, so you can compare band intensities with confidence. You can even conveniently re-image the same blot on the Odyssey CLx later and see the same results.

clx stable signal

NIR fluorescent signals are stable for months, because no enzymes or substrates are used. Direct detection is performed using secondary antibodies labeled with near-infrared fluorescent dyes, such as IRDye® secondary antibodies. You’ll typically lose signal in less than an hour with ECL substrates and indirect enzymatic detection.

clx direct indirect

Compare band intensities confidently with direct detection of stable fluorescent signals.

clx multiplex

See multiple targets on the same blot

With multiplex fluorescence, you can detect two protein targets in each sample lane, with great sensitivity in both fluorescence channels.

Use secondary antibodies labeled with spectrally-distinct NIR fluorescent dyes to get more data from your blot. View, adjust, and analyze your results as a merged image, or as separate 700 nm and 800 nm channel images in pseudo-color or grayscale. Consistency and reproducibility are improved because you can account for lane-to-lane variation in loading and transfer without stripping and re-probing.

“The infrared imaging system allows detection of multiple proteins on a single blot, without the need for stripping and re-probing.”

Bond et al (2008) Biol Proced Online. 10: 20–28.
700nm and 800nm Western Blot

See your strong bands accurately

Get deeper data capacity with digital fluorescence and the unprecedented linear dynamic range of the Odyssey CLx, and never lose data because of image saturation.

clx saturation

Never lose your data because of image saturation. Digital fluorescent detection with the Odyssey CLx shows your strong signals as they really are. Film and CCD imagers only show you part of the picture, because data are lost when images become saturated. To see the whole picture, you need enough capacity (dynamic range) to consistently document your strongest bands.

“When saturated, film exposures can also hide sample-to-sample variations in high-abundance proteins.”

Janes (2015) Science Signaling 8(371): rs2
 
Film has a shallow data capacity and cannot accurately record strong signals. Once that capacity is reached, all additional signal (read: your data) is simply lost. Many CCD imagers are also limited by data capacity and image saturation.
data illustration

Acquisition and analysis software for the Odyssey CLx

Pair your Odyssey CLx Imager with Image Studio Software for streamlined image acquisition and organization and with new Empiria Studio Software for analysis of your Western blots.

Empiria Studio

Quick image acquisition and organization with Image Studio Software

Image Studio Software can be used for quick acquisition and organization of images from most LI-COR imaging systems, including the Odyssey CLx. The simple interface puts high-quality data at your fingertips with a few simple clicks and without manual camera adjustments or multiple exposures.

Learn More about Image Studio

Empiria Studio

Expert Western blot analysis made simple with Empiria Studio Software

Designed for the analysis of near-infrared Western blot images acquired on an Odyssey imager, Empiria Studio is the only Western blot analysis software that guides you all the way from validation to statistical analysis for reproducible results. Streamlined workflows minimize user-to-user variation, compute statistical values, and provide extensive analysis options.

Learn More about Empiria Studio

Odyssey CLx Imaging System

See the Odyssey CLx in action

Do more with consistent, reproducible digital imaging technology that has propelled the Odyssey imager family to over 10,000 peer-reviewed publications. See the benefits of stable NIR fluorescence and detect multiple targets in the same lane. Get the most accurate results with over 6 logs of linear dynamic range, so you can make more discoveries.

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