Empiria Studio | Expert Analysis Made Simple.
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Empiria Studio® Software

Expert analysis made simple.

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Version 3.2 now available. What's new?

At every step, Empiria Studio Software provides intuitive tools to translate great data into meaningful information.

Validate, Analyze, Replicate, and Share

The only software to provide answers at every step for reliable, publishable results.

Execute fast, advanced analysis of assay images acquired using LI-COR imagers—including In-Cell Western™ Assays, Western blots, tissue sections, and small animal images.

Determine target protein expression, build a library of validated antibodies, compare replicates, perform in-depth statistical analysis, share results, and much more—all in one software.

Data images from assays that can be analyzed in Empiria Studio
Assay Analysis Type
Qualitative Western Blots Quick signal identification data and charts
Quantitative Western Blots Comprehensive projects with validation experiments and target analysis, including fold change and statistical calculations
Gels Qualitative and quantitative analysis of protein and nucleic acid gels
Multiwell Plates Comprehensive In-Cell Western, ELISA, and other multiplex fluorescence cell analysis assays
Slides Quantify, examine, organize, and annotate slide images
Small Animal Quantify, examine, organize, and annotate small animal images

No More Analysis Paralysis

Let Empiria Studio handle the complex math for fast, stress-free analysis.

Empiria Studio takes the guesswork out of quantification. Target protein data is automatically normalized based on your chosen internal loading control, and advanced statistical values are automatically calculated, including % CV, standard deviation, and signal-to-noise ratio.

At the end of a target analysis, Empiria Studio calculates fold change and creates a chart using your data.

Empiria Studio Software Calculations Screen
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Created with High-Impact Journals for Replicable Results

Use integrated grant and publisher best practices as a strong foundation for credible imaging results.

Empiria Studio incorporates NIH Guidelines for Scientific Record Keeping and includes Western blot workflows created in partnership with high-impact journals.* This ensures your results meet publication requirements and conform to accepted standards.

*Journal of Biological Chemistry, Nature Publishing Group, Rockefeller University Press

Adaptive Lane Finding

Get more consistent results faster with patented lane finding.

Compared to manual methods, Adaptive Lane Finding accelerates analysis and eliminates variability from manual lane placement. Analyze up to 26 lanes and multiple channels with up to two targets per channel.

In conjunction with Adaptive Background Subtraction, Adaptive Lane Finding improves total protein stain quantification to maximize the reliability of your Western blot results.

Graph for adaptive lane finding with Empiria Studio was more consistent than local and global background-correction
Data image for adaptive lane finding with Empiria Studio was more consistent than local and global background-correction
Quantitative analysis with Empiria Studio was more reproducible than manual and traditional lane finding methods. In a comparative study, nine researchers experienced in traditional Western blot analysis quantified lanes from six Western blot images using different lane finding methods. Variability of background-corrected lane intensity, expressed as the mean % CV of the quantitative results for each lane, was compared for each lane finding method. The mean for each method is represented by a black horizontal bar. Adapted from the Adaptive Western Blot Analysis White Paper.

Adaptive Background Subtraction

Quantification in Empiria Studio uses a patented background subtraction method optimized for consistency.

Adaptive Background Subtraction adjusts to the unique background in each image and subtracts background automatically. Because users do not need to choose a background subtraction method, variability from user choice is eliminated and results among multiple researchers are consistent.

Graph for Quantitative analysis with Empiria Studio was more consistent than local and global background subtraction
Data image for Quantitative analysis with Empiria Studio was more consistent than local and global background subtraction
Quantitative analysis with Empiria Studio was more consistent than local and global background subtraction. Multiple users analyzed the designated bands (arrowheads on the image) using local, global/user-defined, and Adaptive Background Subtraction. Variability of background-corrected band intensity, expressed as the mean % CV of the quantitative results for that image, was compared for each background subtraction method. In this figure from the Adaptive Western Blot Analysis White Paper, faint bands were surrounded by background in sample lanes. The graph shows the mean CV for each method (error bars show standard deviation, n = 6).

Validation is a Great Foundation

Empiria Studio uses intuitive analysis workflows for consistent image analyses among all users.

Empiria Studio Software antibody validation screen

Antibody Validation

Confirm that a primary antibody is specific and selective to its expected target using the Antibody Validation workflow.

Empiria Studio Software combined linear range determination screen

Combined Linear Range Determination

Use the Combined Linear Range Determination workflow in every membrane-based experiment; otherwise, it can be difficult to load the correct protein amount and accurately measure changes in protein expression.

Empiria Studio Software antibody validation Housekeeping protein validation screen

Housekeeping Protein Validation

When using a housekeeping protein as an internal loading control, it is important to ensure that its expression is not altered by conditions in the experiment. Use the Housekeeping Protein Validation workflow for verification.

Save Time, Improve Consistency with Reusable Multiwell Plate Templates

Plan experiments and analyze results correctly—every time.

Create plate templates to plan the experimental layout of your multiwell plate assays, such as In-Cell Western Assays or ELISAs. Then print the template and place it under your plate to see exactly which wells to load with controls, background, and treatments.

By using the same template to load your wells and analyze images, you can track experimental details and achieve reliable results by ensuring well loading and analysis are consistent.

Empiria Studio Experiment Designer
List of Experiments in Empiria Studio Software

Start Strong and Stay Organized

Easily analyze and organize images using Projects.

Project-based organization preserves the integrity of your data by keeping all the parts of your experiments together. Experiment files contain all images, analysis details, and quantification data.

This approach makes quickly finding and sharing experimental data and related analysis easy and transparent.


Replicate, Replicate, Replicate

Compare replicates to clearly demonstrate the significance of your data.

Compare replicates to determine fold change, percent change, standard deviation, and other statistics. This makes it simple to consolidate the data you care about in a single software package—without the uncertainty and hassle of formulas and spreadsheets.

Empiria Studio Software Scatter plot screen
Empiria Studio Software publish and share illustration

Publish and Share with Everyone

Streamline file and data sharing among colleagues, labs, and publishers.

Empiria Studio makes it simple to share all your images, supporting data, and target analysis data in just one file.

  • Export PDF reports with images, data tables, graphs, acquisition details, and Adobe® Sign electronic signatures.
  • Export raw data as a CSV file or as an Excel spreadsheet.
  • Export images and graphs in the format that publishers require.
  • Export a Project’s content—such as images, data, experimental details, and results—as a single file that you can quickly share with colleagues who have Empiria Studio.

Save on Your Empiria Studio License Today

Save money on Empiria Studio when you buy a license for yourself or your whole lab.

License Terms

  1. An Empiria Studio license provides you with lifetime access to Empiria Studio Software and includes updates, upgrades, and fixes for one year.
  2. After the first year, you may purchase an annual license renewal at a reduced cost for continued access to updates, upgrades, and fixes.

Reviewer and Editorial Licenses

If you are currently a reviewer or part of the editorial staff at a scientific journal, contact us to get your free license.

Empiria Studio Software Price Purchasing
9141-500 1 User License $305.00 305.00 € £278.00 Order Online
9141-505E 5 User Licenses $1,148.00 1.148,00 € £1,044.00 Contact Us
9141-507E 10 User Licenses $1,913.00 1.913,00 € £1,740.00 Contact Us
9141-510E 25 User Licenses $3,825.00 3.825,00 € £3,479.00 Contact Us

Empiria Studio requires a minimum of 8 GB of RAM. 64-bit versions of Windows 10 and Windows 11 are supported. macOS Ventura and Sonoma are supported on both Intel and Apple Silicon processors.

Ready for Reliable Results?