Empiria Studio Software. Expert Analysis Made Simple.
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Empiria Studio® Software

Expert analysis made simple.

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Empiria Studio Software is a post-processing, Data Integrity Software for quantitative protein expression analysis—including In-Cell Western™ Assays, Western blots, membranes, slides, and gels.

Unlike signal identification software such as Image Studio™ Software, it provides workflows that are designed to walk users of all experience levels through each stage—from validation and analysis to replicability and publication—of image post processing. These workflows ensure that all users consistently analyze data that are captured with the Odyssey® Imager.


Outperform with Data Integrity Software

Execute faster, better data analysis among users with all levels of experience.

Signal identification software—such as Image Studio™ Software—only identifies an image’s signal levels. As a Data Integrity Software, Empiria Studio Software outperforms basic signal identification and provides expert data analysis for all users. Key factors, such as the incorporation of publishers’ best practices and systematic workflows, make Empiria Studio faster, more reliable, and consistent for every lab member.

To prove it, LI-COR employees—with Beginner, Intermediate, and Expert levels of experience—conducted a comparison study. When each group analyzed the same Western blot data, they collectively achieved a significantly lower % CV with Empiria Studio than with the signal identification software and completed their analyses faster.

Here's how our employees did

Employee results for signal identification software: Beginner: 18% CV, 126 min; Intermediate: 10% CV, 93 min; Expert: 10% CV, 93 minutes. Employee results for Empiria Studio Software: Beginner: 3% CV, 35 min; Intermediate: 3% CV, 37 minutes; Expert: 3% CV, 38 minutes. Try it for Yourself
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Created with High Impact Journals for Authentic Results

Use integrated grant and publisher best practices as a strong foundation for credible imaging results.

Empiria Studio is created with incorporated NIH Guidelines for Scientific Record Keeping and in partnership with high impact journals*—ensuring that publication requirements are met when using Empiria Studio. Together, these new features and integrated publisher recommendations verify that your analysis and results conform to accepted Western blotting standards.

*Journal of Biological Chemistry, Nature Publishing Group, Rockefeller University Press

Validate, Analyze, Replicate, and Share

Walk through easy-to-follow workflows to generate reliable and publishable data images.

Empiria Studio is the only software that provides reliable answers at every step of the process. With its workflows, users can analyze validation blots, determine target protein expression, compare replicates, perform statistical analysis, and share results using just one Data Integrity Software package.

Empiria Studio offers step-by-step workflows for various experimental processes, such as the Western blot, to ensure that each user’s results are altogether reliable and consistent.

Assay Analysis Type
Qualitative Western Blots Quick signal identification data and charts
Quantitative Western Blots Comprehensive projects with validation experiments and target analysis, including fold change and statistical calculations
Gels Qualitative and quantitative analysis of protein and nucleic acid gels
Multiwell Plates Comprehensive In-Cell Western and other multiplex fluorescence cell analysis assays
ELISA Quick signal quantification of plate images
Slides Examine, organize, and annotate slide images
Empiria Studio Software Workflow

Automatic Lane Detection

Find lanes automatically in Empiria Studio
with a lane detection method.

When using Adaptive Lane Finding (ALF) instead of manual methods, analysis is accelerated and variability from manual lane placement is completely eliminated. With this automation, user-to-user consistency and data reliability are also greatly improved. Analyze up to 26 lanes and multiple channel images with up to two targets per channel. ALF works in conjunction with Adaptive Background Subtraction (ABS) to improve total protein stain quantification and help produce reliable Western blot results.

Graph for adaptive lane finding with Empiria Studio was more consistent than local and global background-correction
Data image for adaptive lane finding with Empiria Studio was more consistent than local and global background-correction
Quantitative analysis with Empiria Studio was more reproducible than manual and traditional lane finding methods. In a comparative study, nine different researchers quantified lanes from six Western blot images using a variety of lane finding methods. Researchers were all experienced in traditional Western blot analysis. Variability of background-corrected lane intensity, expressed as the mean % CV of the quantitative results for each lane, was compared for each lane finding method. The mean for each method is represented by a black horizontal bar. Adapted from the Adaptive Western Blot Analysis White Paper.

Background Subtraction

Quantification in Empiria Studio uses a background subtraction method optimized for consistency.

Adaptive Background Subtraction (ABS) adjusts to the unique background in each image and subtracts background automatically. Because the user does not need to choose a background subtraction method, variability caused by subjective background subtraction choices is eliminated, and results among multiple researchers are consistent.

Graph for Quantitative analysis with Empiria Studio was more consistent than local and global background subtraction
Data image for Quantitative analysis with Empiria Studio was more consistent than local and global background subtraction
Quantitative analysis with Empiria Studio was more consistent than local and global background subtraction. Multiple users analyzed the designated bands (arrowheads on the image) using local, global/user-defined, and Empiria Studio Adaptive Background Subtraction. Variability of background-corrected band intensity, expressed as the mean % CV of the quantitative results for that image, was compared for each background subtraction method. In this figure from the Adaptive Western Blot Analysis White Paper, faint bands were surrounded by background in sample lanes. The graph shows the mean CV for each method (error bars show standard deviation, n = 6).
Empiria Studio Experiment Designer

Create a Digital Template for Each Cell-Based Experiment

Design an assay plate map to set up experiments and analyze results correctly every time.

The Empiria Studio Experiment Designer is a digital template that models and organizes a plate’s experimental layout—allowing users to visualize exactly which wells contain the positive controls and negative controls, background, and treatments.

The Experiment Designer lets you plan plate templates used in your cell-based assays—such as In-Cell Western Assays and ELISAs. Templates created with the Experiment Designer are used for consistent well loading in the lab and consistent analysis in Empiria Studio. By planning a well layout and comparing the layout with acquired images, the Experiment Designer helps you track your experiment and ensure that everything is correctly loaded and analyzed.

List of Experiments in Empiria Studio Software

Start Strong and Stay Organized

Easily start analyzing images using the Project feature.

Project-based organization makes quickly finding and sharing experimental data and related validation data easy. Empiria Studio even notifies you if a Project is missing publisher-required validation data.


Validation is a Great Foundation

Empiria Studio has easy-to-follow analysis workflows for consistent image analyses among all users.

Empiria Studio Software antibody validation screen

Antibody Validation

Confirm that a primary antibody is specific and selective to its expected target using the Antibody Validation workflow.

Empiria Studio Software combined linear range determination screen

Combined Linear Range Determination

Use the Combined Linear Range Determination workflow in every membrane-based experiment; otherwise, it can be difficult to load the correct protein amount and accurately measure changes in protein expression.

Empiria Studio Software antibody validation Housekeeping protein validation screen

Housekeeping Protein Validation

When using a housekeeping protein as an internal loading control, it is important to ensure that its expression is not altered by conditions in the experiment. Use the Housekeeping Protein Validation workflow for verification.

Empiria Studio Software Calculations Screen

No More Analysis Paralysis

Lean on automatic techniques in Empiria Studio for a simple and stress-free analysis.

With Empiria Studio, target protein data are automatically normalized (based on the type of internal loading control), and vital statistical values are automatically calculated.

At the end of a target analysis, Empiria Studio calculates fold change and automatically creates a chart with the data. Results are viewable within minutes—eliminating the need for spreadsheets, graphing software, or word processing in order to analyze In-Cell Western Assay and Western blot images.

Replicate, Replicate, Replicate

Compare replicate samples and blots to clearly illustrate the significance of your data.

Empiria Studio enables you to compare replicate samples on the same blot or across multiple blots to determine fold change, percent change, standard deviation, and other statistics. This feature makes it simple to consolidate the data you care about in a single Data Integrity Software package—without the uncertainty and hassle of formulas and spreadsheets.

Empiria Studio Software Scatter plot screen
Empiria Studio Software publish and share illustration

Publish and Share with Everyone

No scientist is an island, so Empiria Studio streamlines the file and data sharing process among colleagues, labs, and publishers.

  • Export PDF reports with images, data tables, graphs, acquisition details, and Adobe® Sign electronic signatures.
  • Export raw data as a CSV file or as an Excel spreadsheet.
  • Export images and graphs in the format that publishers require.
  • Export a Project’s content—such as images, data, experimental details, and results—as a single file that you can quickly share with colleagues who have Empiria Studio.

Save on Your Empiria Studio License Today

Save money on Empiria Studio when you buy a license for yourself or your whole lab.

License Terms

  1. An Empiria Studio license provides you with lifetime access to Empiria Studio Software and includes updates, upgrades, and fixes for one year.
  2. After the first year, you may purchase an annual license renewal at a reduced cost for continued access to updates, upgrades, and fixes.

Reviewer and Editorial Licenses

If you are currently a reviewer or part of the editorial staff at a scientific journal, contact us to get your free license.

Empiria Studio Software Price Purchasing
9141-500E 1 User License $299.00 299.00 € £272.00 Order Online
9141-505E 5 User Licenses $1,125.00 1.125,00 € £1,023.00 Contact Us
9141-507E 10 User Licenses $1,875.00 1.875,00 € £1,705.00 Contact Us
9141-510E 25 User Licenses $3,750.00 3.750,00 € £3,410.00 Contact Us

Empiria Studio requires a minimum of 8 GB of RAM; 64-bit versions of Windows 10; Mac OS Mojave, Catalina, or Monterey.

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