Empiria Studio Software is a post-processing, Data Integrity Software for quantitative protein expression analysis—including In-Cell Western™ Assays, Western blots, membranes, slides, and gels.
Unlike signal identification software such as Image Studio™ Software, it provides workflows that are designed to walk users of all experience levels through each stage—from validation and analysis to replicability and publication—of image post processing. These workflows ensure that all users consistently analyze data that are captured with the Odyssey® Imager.
Execute faster, better data analysis among users with all levels of experience.
Signal identification software—such as Image Studio™ Software—only identifies an image’s signal levels. As a Data Integrity Software, Empiria Studio Software outperforms basic signal identification and provides expert data analysis for all users. Key factors, such as the incorporation of publishers’ best practices and systematic workflows, make Empiria Studio faster, more reliable, and consistent for every lab member.
To prove it, LI-COR employees—with Beginner, Intermediate, and Expert levels of experience—conducted a comparison study. When each group analyzed the same Western blot data, they collectively achieved a significantly lower % CV with Empiria Studio than with the signal identification software and completed their analyses faster.
Use integrated grant and publisher best practices as a strong foundation for credible imaging results.
Empiria Studio is created with incorporated NIH Guidelines for Scientific Record Keeping and in partnership with high impact journals*—ensuring that publication requirements are met when using Empiria Studio. Together, these new features and integrated publisher recommendations verify that your analysis and results conform to accepted Western blotting standards.
*Journal of Biological Chemistry, Nature Publishing Group, Rockefeller University Press
Walk through easy-to-follow workflows to generate reliable and publishable data images.
Empiria Studio is the only software that provides reliable answers at every step of the process. With its workflows, users can analyze validation blots, determine target protein expression, compare replicates, perform statistical analysis, and share results using just one Data Integrity Software package.
Empiria Studio offers step-by-step workflows for various experimental processes, such as the Western blot, to ensure that each user’s results are altogether reliable and consistent.
| Assay | Analysis Type |
|---|---|
| Qualitative Western Blots | Quick signal identification data and charts |
| Quantitative Western Blots | Comprehensive projects with validation experiments and target analysis, including fold change and statistical calculations |
| Gels | Qualitative and quantitative analysis of protein and nucleic acid gels |
| Multiwell Plates | Comprehensive In-Cell Western and other multiplex fluorescence cell analysis assays |
| ELISA | Quick signal quantification of plate images |
| Slides | Examine, organize, and annotate slide images |
Find lanes automatically in Empiria Studio
with a lane detection method.
When using Adaptive Lane Finding (ALF) instead of manual methods, analysis is accelerated and variability from manual lane placement is completely eliminated. With this automation, user-to-user consistency and data reliability are also greatly improved. Analyze up to 26 lanes and multiple channel images with up to two targets per channel. ALF works in conjunction with Adaptive Background Subtraction (ABS) to improve total protein stain quantification and help produce reliable Western blot results.
Quantification in Empiria Studio uses a background subtraction method optimized for consistency.
Adaptive Background Subtraction (ABS) adjusts to the unique background in each image and subtracts background automatically. Because the user does not need to choose a background subtraction method, variability caused by subjective background subtraction choices is eliminated, and results among multiple researchers are consistent.
Design an assay plate map to set up experiments and analyze results correctly every time.
The Empiria Studio Experiment Designer is a digital template that models and organizes a plate’s experimental layout—allowing users to visualize exactly which wells contain the positive controls and negative controls, background, and treatments.
The Experiment Designer lets you plan plate templates used in your cell-based assays—such as In-Cell Western Assays and ELISAs. Templates created with the Experiment Designer are used for consistent well loading in the lab and consistent analysis in Empiria Studio. By planning a well layout and comparing the layout with acquired images, the Experiment Designer helps you track your experiment and ensure that everything is correctly loaded and analyzed.
Easily start analyzing images using the Project feature.
Project-based organization makes quickly finding and sharing experimental data and related validation data easy. Empiria Studio even notifies you if a Project is missing publisher-required validation data.
Empiria Studio has easy-to-follow analysis workflows for consistent image analyses among all users.
Confirm that a primary antibody is specific and selective to its expected target using the Antibody Validation workflow.
Use the Combined Linear Range Determination workflow in every membrane-based experiment; otherwise, it can be difficult to load the correct protein amount and accurately measure changes in protein expression.
When using a housekeeping protein as an internal loading control, it is important to ensure that its expression is not altered by conditions in the experiment. Use the Housekeeping Protein Validation workflow for verification.
Lean on automatic techniques in Empiria Studio for a simple and stress-free analysis.
With Empiria Studio, target protein data are automatically normalized (based on the type of internal loading control), and vital statistical values are automatically calculated.
At the end of a target analysis, Empiria Studio calculates fold change and automatically creates a chart with the data. Results are viewable within minutes—eliminating the need for spreadsheets, graphing software, or word processing in order to analyze In-Cell Western Assay and Western blot images.
Compare replicate samples and blots to clearly illustrate the significance of your data.
Empiria Studio enables you to compare replicate samples on the same blot or across multiple blots to determine fold change, percent change, standard deviation, and other statistics. This feature makes it simple to consolidate the data you care about in a single Data Integrity Software package—without the uncertainty and hassle of formulas and spreadsheets.
No scientist is an island, so Empiria Studio streamlines the file and data sharing process among colleagues, labs, and publishers.
Save money on Empiria Studio when you buy a license for yourself or your whole lab.
License Terms
Reviewer and Editorial Licenses
If you are currently a reviewer or part of the editorial staff at a scientific journal, contact us to get your free license.
| Empiria Studio Software | Price | Purchasing | ||||
|---|---|---|---|---|---|---|
| 9141-500E | 1 User License | $299.00 | 299.00 € | £272.00 | Order Online | |
| 9141-505E | 5 User Licenses | $1,125.00 | 1.125,00 € | £1,023.00 | Contact Us | |
| 9141-507E | 10 User Licenses | $1,875.00 | 1.875,00 € | £1,705.00 | Contact Us | |
| 9141-510E | 25 User Licenses | $3,750.00 | 3.750,00 € | £3,410.00 | Contact Us | |
Empiria Studio requires a minimum of 8 GB of RAM; 64-bit versions of Windows 10; Mac OS Mojave, Catalina, or Monterey.
