Odyssey® Chemifluorescent Substrate Kit (Nitro)
Odyssey® Chemifluorescent Substrate Kit (PVDF)
Odyssey® Chemifluorescent Substrate
Do you have a few stubborn protein targets that are especially challenging to detect using near-infrared dye-labeled secondary antibodies? When you use the Odyssey Chemifluorescent Substrate Kits, you can detect these challenging targets.
With the Odyssey Chemifluorescent Substrate, you can get the benefits of near-infrared fluorescence detection with few changes to your chemiluminescent protocol. The substrate is oxidized by horseradish peroxidase (HRP) from the HRP-conjugated secondary antibodies, generating a precipitate that fluoresces and can be detected in the 700nm channel of Odyssey Imaging Systems.
Odyssey Chemifluorescent Substrate Kits:
When using chemifluorescent substrates, avoid using Odyssey blocking buffers or any other blocking buffers or antibody diluents that contain sodium azide, as it inhibits HRP activity.