Image Tab

The Image tab has controls for zooming, viewing, copying, rotating or flipping, reducing noise, aligning channels, and cropping an image. Selecting any of the options in the Create group creates a new image. Any analysis of the image is not copied. You can also specify a print/export area or quickly copy the image or a region of the image to the system clipboard.

Zoom

There are several zoom controls for zooming the image on the current analysis:

• Zoom In — Zooms in on the image. The zoom view is centered on the current pointer position on the image. Press Zoom In again to zoom in another increment.
• Zoom Out — Zooms out on the image. Press Zoom Out again to zoom back out another increment.
• Zoom Selection — Zooms to the highest zoom that will display all selected features so that all the features are visible.
• Restore — Restores the zoom to fit the window.
• Zoom by % — Selects a zoom value for the current image view.

Adjusting the mouse wheel over the image will also zoom in or zoom out.

Display

Click Choose Display and six display options with varying intensities are displayed. Click the desired display.

Click Adjust Display to adjust how the current image is displayed. Select a channel to adjust and the current image is displayed in that channel, along with brighter and dimmer options. Adjust the signal, background, or midtones. Adjust the midtones to change the mapping from linear to logarithmic. This 'compression' of the intensity values may improve the appearance of less intense bands while avoiding overly bright bands.

View Mode

The View Mode lets you specify the view mode to use for displaying images. Select either the editable Single Image View mode or the Multiple Image View mode to display images in the main application window. Adjust display parameters for these images using the Display Panel (see Display for details.)

• Single — Shows a single image that is fully editable. Any image in the Images Table can be displayed in an image view.
• Multiple — Shows several images from the Images Table. If there are a large number of images in the Images Table, only a subset of the images may be displayed. No edits can be made directly on these views. Use the drop-down menu to choose how many images to display horizontally.

Slide Show

Click Start and the images in the Table will appear in the window sequentially. Click Stop to return to normal viewing. Click the arrow on the Slide Show group to open the Slide Show Parameters dialog. Choose to display the images in the Table and stop at the end, or return to the beginning of the table and continue displaying the images. Adjust the slider to modify the amount of time each image is displayed.

Adjust Display

The Adjust Display dialog helps you set the min and max points of the display mapping. These settings affect the display of the image only. They do not affect the data or quantification of the data. See Image Display Panels for more information.

Click Adjust in the Display group on the Image tab to open the Adjust Display dialog.

You can also click the Adjust Display button () on the Display panel on the right side of the window.

The Choose Display and Adjust Display dialogs automatically open after a scan for each channel acquired. Click the arrow (>) in the Display group to open the Display Options dialog and remove the check marks to disable this feature.

The middle tile displays the image with the current settings. Click the tile to the left or the right to change the settings to that display. You can also click Dimmer or Brighter below to change the settings.

Click Done to close the dialog. The image will be displayed with the settings from the middle tile. To close the dialog without changing the settings for the display, click Cancel at the bottom right. Reset the display in the dialog to the original settings by clicking Reset.

Choose the settings to adjust at the bottom left of the dialog. Click Signal, Background, or Midtones.

Choose Display

The Choose Display dialog allows you to choose the best display of the image from six options. This dialog affects the display of the image only. It does not affect the data or quantification of the data. See Display for more information.

Click Choose in the Display group on the Image tab to open the Choose Display dialog. Select the channel to display.

You can also click the Choose Display button () on the Display panel.

The Choose Display and Adjust Display dialogs automatically open after a scan for each channel acquired. Click the arrow (>) in the Display group to open the Display Options dialog and remove the check marks to disable this feature.

Click the tile that best displays the data. The Adjust Display dialog will then open to allow further adjustment of the display. You can disable this feature by removing the check mark by Automatically launch the Adjust Display dialog at the bottom right of the Choose Display dialog.

Copy Image

Copy Image makes a copy of the original image (without the analysis) and gives it a new name. You will get a message that tells you that the copy was successful and the name of the new image.

Rotate or Flip

Rotate or Flip Image creates a copy of the image and then displays the choices for rotation or flipping.

1. Select the image that you want to rotate or flip.

2. Click Rotate or Flip. The Rotate or Flip Options dialog is displayed.

   

3. Choose the direction to rotate or flip. The new image is displayed.

Free Rotate

Free Rotate creates a copy of the image and then displays the choices for rotating.

1. Select the image that you want to rotate.
2. Click the Free Rotate. The Free Rotate Options dialog is displayed.



3. Use the Rotate buttons to rotate left or right. Repeat until the image is rotated as needed. Click OK and the new rotated image is displayed.

Reduce Noise

Reduce Noise creates a new image and reduces the noise on that image.

1. Select the Image.

2. Click Reduce Noise. The Reduce Noise Options dialog is displayed.

   

3. Noise Removal — Removes salt & pepper noise using a median filter from 3x3 closest pixels
4. Smooth — Enhances the image using the local average from 3x3 closest pixels
5. Local Maximum — Enhances the image using the local maximum from 3x3 closest pixels.
6. Sharpen — Enhances contrast at image boundaries using 3x3 closest pixels
7. Local Minimum — Enhances the image using the local minimum from 3x3 closest pixels
8. Click OK. A new image is created and noise on the new image is reduced.

Align Channels

Align Channels displays a window where the placement of one channel can be adjusted in reference to the other channel. A new image is created when the OK button is pressed.

This action cannot be undone once the OK button is clicked.

1. Select the image that has channels that you want to align.

2. Click the Align Channels on the Image tab. The Align Channels dialog is displayed.

   

3. Select the Reference Channel from the drop-down menu. This channel will not move.
4. Use the up or down arrows to move the other channel(s) to the top or bottom, or use the bars on either side of the arrows to move the other channel(s) left or right.
5. Click OK. A new image is created with the channels aligned.

Crop

The Crop tools define an area of the display for exporting and printing as well as for cropping. You can crop the image by clicking Crop, or define an area for exporting and printing single images.

If you crop the image, a new image of the cropped area is created. The original image remains unchanged.

Modify the Crop Area

This action is used to define an area to display when exporting or printing an image for publication.

1. Display the image that you want to define.

2. Click the Modify in the Print/Export Area group. The crop area is outlined in a dotted line with a box on each corner and a box in the center. The Modify Image Print/Export Area dialog is also displayed. You may need to move the dialog if it is blocking the view of the crop area.

   You can change the color of the crop outline while it is selected by clicking Edit Color... at the top right of the dialog.



• Click the box in the center to move the entire crop area by using the four-pointed arrow to drag it to the area you want cropped.
• Click one of the boxes in a corner to resize the crop area by dragging the double arrow (up or down or right or left).

3. When you have set the crop marks the way you want, select whether to apply the crop area to the current image or to all the images in the Images table. Select Only adjust size and the same crop area will be applied to all of the images. Select Adjust size and location to apply the same crop area in the same location on all the images.

   

4. Click OK. Click Apply in the Image tab to highlight the button. The crop area is defined for printing or exporting. The image is not cropped.

Crop the Image

The Crop button uses the crop marks on the current image to create a new cropped image. You can modify the crop marks first.

1. Click the Crop button in the Create group. The Crop to New Image dialog is displayed and the crop area is outlined in a dotted line with a box on each corner and a box in the center. Adjust the crop area in the same ways as described in Define the Crop Area above.



2. Click OK. A new image of the cropped area is created.

Image Formats

Image Studio Software can import and export TIFF, JPEG, and PNG images.

TIFF File Format

Use TIFF images for applications that require the highest fidelity. Usually, TIFF images at 300 or 600 dots per inch (dpi) are appropriate for journal publication.

JPEG File Format

When a JPEG image is exported, an algorithm creates an image that appears like the original but is "compressed" to use less file space. The smaller file size makes JPEG images useful for web pages, presentation software, and general printing (e.g. a lab notebook).

Do not quantify JPEG images. The image data have been altered in the export process.

Image Studio Softwareuses a moderate JPEG compression that may cause a "halo" to appear around bands.

PNG File Format

PNG images are created using an algorithm that compresses the original image to take up less file space without any loss of information. The smaller file size makes PNG images useful for applications such as web pages, presentation software, and general printing (e.g. a lab notebook).