Western Blot Stripping Buffers Effectively Remove Antibodies

Western Blot Stripping Buffers


LI-COR Stripping Buffers Help You Conserve Precious Samples and Time

Stripping and reprobing a Western blot is a common laboratory method. Many labs choose to make their own stripping buffer. With pre-made LI-COR Western blot stripping buffers, you can:

  • Conserve precious samples by re-using the same blot up to three times to detect different targets or optimize antibody concentrations
  • Save time and money
  • Effectively remove antibodies, while retaining immobilized proteins
  • Strip blots at room temperature in 20 minutes or less without unpleasant odor
  • Perform qualitative analysis after stripping

NewBlot Stripping Buffers are optimized for stripping and reprobing near-infrared (NIR) fluorescent Western blots, including those detected with IRDye® secondary antibodies.

WesternSure® ECL Stripping Buffer is optimized for stripping and reprobing chemiluminescent Western blots.

Which Stripping Buffer Should I Choose?

Product Membrane Compatibility Type of Blot Performance on Nitrocellulose Performance on PVDF Hazardous Shipping
*WesternSure> ECL Nitro/PVDF Chemiluminescence checkmark checkmark
*NewBlot IR Nitro/PVDF Near-infrared fluorescence checkmark** checkmark
NewBlot Nitro Nitro Near-infrared fluorescence checkmark checkmark
NewBlot PVDF PVDF Near-infrared fluorescence checkmark checkmark
*NewBlot IR Stripping Buffer and WesternSure ECL Stripping Buffer are not recommended for loading amounts over 30 μg.
**Performance is target-dependent. For some targets it works as well as NewBlot Nitro, for others it is not as effective.