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To keep your targeted therapeutics moving through the development process, you need to be able to make decisions with confidence, and without hesitation or costly delays. Whether you’re characterizing a signaling pathway or validating a therapeutic candidate, confident decisions begin with exceptional data.
Get the answers you depend on to keep the development process moving forward. Odyssey® Imagers and Pearl® Trilogy Small Animal Imagers deliver superior data for robust, replicable results you can rely on.
IRDye® Reagents are optimized for use with Odyssey Imagers and the Pearl Trilogy Imagers to provide an additional layer of certainty.
Characterize your cell signaling pathways with confidence using quantitative, near-infrared (NIR) Western blots performed on an Odyssey Fc or Odyssey CLx Imager and quantitative, NIR In-Cell Western™ or On-Cell Western assays performed on an Odyssey CLx. Gain a fuller understanding of the details of your ligands, targets, pathways, and cellular effects.
Quickly screen targets and identify potential therapeutic leads against specific pathways with DigiWest® Protein Profiling Services. Then, perform cell signaling assays on the Odyssey CLx.
Assess your therapeutic agent’s performance in vitro and ex vivo using the Odyssey CLx Imager or in vivo and ex vivo using the Pearl Trilogy Small Animal Imager. Decide which of your candidates appears most promising and move to the next step of validation.
Understand more about your targets, pathways, and the effects your therapeutic has when you perform cell signaling assays on an Odyssey CLx Imager.
Use the IC50 assay with the Odyssey CLx and IRDye Reagents to find the concentration of an inhibitor required to reduce a response by half. Get the data you need to continue through discovery and development.
PTK6 is abnormally expressed in breast cancers, and researchers have described kinase-dependent functions of PTK6 in driving tumor growth. As with any therapeutic approach, using small molecule inhibitors to target PTK6 activity requires validation of specificity and efficacy.
With DigiWest® Protein Profiling Services offered by NMI TT Pharmaservices through LI-COR, you can rapidly screen over 1,000 prevalidated antibodies against 50+ predefined signaling pathways. This will quickly narrow down your targets for further characterization against your therapeutic and for examination using an In-Cell Western Assay or Western blot.
Proteins are size separated via SDS-PAGE.
Proteins are transferred to a blotting membrane and biotinylated.
Sample lanes are cut into 96 strips to generate 96 molecular weight fractions immobilized on membrane.
Proteins are eluted into 96-well plates.
Biotinylated proteins are immobilized on the surface of Neutravidin-coated Luminex beads. One distinct bead set is added to each of the 96 wells, which results in a collection of distinguishable protein-loaded bead sets from a single sample.
Beads are pooled to reconstruct the initial sample lane where a defined color code correlates with each molecular weight of the immobilized proteins.
Immunoassay: a small aliquot of the bead pool is incubated with Western blot antibodies before Phycoerythrin-labeled secondary antibodies are added for signal generation.
The sample is detected on a Luminex FLEXMAP 3D® instrument.
DigiWest® profile of protein analytes is generated.
From advanced analysis of vetted therapeutic leads to small animal, tissue, and organ imaging, we offer solutions to help you understand the key characteristics of your therapeutic before moving to the clinic.
The Odyssey CLx Imager and the Pearl Trilogy Small Animal Imager provide an ideal combination to assess therapeutic delivery with NIR fluorescence and for complete analysis of your therapeutic agent’s performance.
The therapeutic agents tagged with IRDye Reagents deliver a clear image for detection and quantification. Validate your therapeutics and probes in vitro and ex vivo with the Odyssey CLx or in vivo and ex vivo with the Pearl Trilogy Small Animal Imager.
Learn how to maximize your targeted therapeutics research and make groundbreaking scientific advancements with NIR detection and innovative probe development.
Ovarian and uterine cancers are often diagnosed at advanced stages, underscoring the need to identify therapeutic targets and means of delivering therapies to tumors. One promising therapeutic target is the AXL protein, which is involved in ovarian and uterine cancer metastasis. One potential treatment option is to silence AXL expression with siRNA, but a mechanism is needed to deliver the siRNA to the tumor.
Characterize and validate the specificity, binding affinity, and other parameters of your therapeutic agents with the In-Cell Western or On-Cell Western assay performed on the Odyssey CLx Imager. Get the confidence you need in your agents before moving forward with small animal and tissue section imaging.
Capture a detailed representation of the in vivo molecular activity of your therapeutic agents in live animals. Assess uptake, clearance, and other key factors for development.
Use an Odyssey CLx or Pearl Trilogy Imager to quantify the presence of dye-tagged probes in excised tissue or organ samples to assess the biodistribution, clearance, and other parameters of your therapeutic leads.