PDF Documents

Summary
Dye Decontamination Kit Pack Insert (P/N 9300-840, 9300-841, 9300-842)This decontamination kit effectively and safely removes dye contamination from the skin of the animal or surface of Pearl imaging beds. Pack Insert
MPX Blotting System User GuideThis User Guide explains how to use the MPX Blotter accessory.Manual
WesternSure Pen Quick Reference CardUse instructions for the WesternSure Pen (P/N 926-91000).Quick Card
Quick Start Guide for the C-DiGit Blot ScannerThis Quick Start Guide explains how to start using Image Studio Software version 5.0 with the C-DiGit Blot Scanner.Quick Start
Install Guide for the C-DiGit Blot Scanner Windows 10 DriverIf you are using Windows 10, a separate driver must be installed to connect your C-DiGit Blot Scanner to Image Studio Software via USB.Installation Guide
C-DiGit Quick Start GuideThis Quick Card covers installing the C-DiGit Blot Scanner and Image Studio Software 5.0 for the C-DiGit.Quick Card
C-DiGit Blot Scanner Operator's ManualOperator Manual for the hardware specifications and operations of the C-DiGit Blot Scanner.Manual
Image Studio Software 5.x.x for the C-DiGit Blot Scanner Installation Instructions This installation guide explains how to install the C-DiGit Blot Scanner with Image Studio Software 5.x.x.Installation Guide
Cleaning and Shipping Instructions for the D-DiGit Gel ScannerThis guide explains how to clean and package the D-DiGit Gel Scanner for shipping.Quick Start
Quick Start Guide for the D-DiGit Gel ScannerThis guide explains how to setup and begin using the D-DiGit Gel Scanner.Quick Start
D-DiGit Gel Scanner User GuideOperator Manual for the D-DiGit Gel Scanner. Includes hardware specifications.Manual
Odyssey DLx Imaging System Operator's ManualOperator's Manual for the Odyssey DLx Imaging System. Includes hardware specifications.Manual
Odyssey DLx Operator’s Manual for Labs Using Image Studio 21 CFR Part 11Operator's Manual for labs using Image Studio 21 CFR Part 11 Software with the Odyssey DLx Imager.Manual
Moving Instructions for Odyssey DLx and CLx ImagersInstructions for moving the Odyssey DLx or Odyssey CLx Imager within the lab.Quick Start
Odyssey M Imaging System Operator's ManualOperator's Manual for the Odyssey M Imaging System. Includes hardware specifications.Manual
Odyssey XF Operator's ManualOperator's Manual for the hardware specifications and operations of the Odyssey XF Imaging System.Manual
Odyssey XF Operator’s Manual for Labs Using Image Studio 21 CFR Part 11Operator's Manual for labs using Image Studio 21 CFR Part 11 Software with the Odyssey XF Imager.Manual
Odyssey Fc Imaging TrayP/N 926-40020Accessory Insert
Pack the Odyssey Fc Imager for ShippingFollow these steps to pack the Odyssey Fc into its shipping case.Quick Start
Moving Instructions for Odyssey XF and Fc ImagersInstructions for moving the Odyssey XF or Odyssey Fc Imager within the lab.Quick Start
Pearl Macroscopic Imaging SystemPearl Macroscopic Imaging System ManualManual
Image Studio Software 5.x.x Installation Instructions for Pearl Imaging SystemsInstall Image Studio 5.x for use with a Pearl Imaging System.Installation Guide
Pearl Accessories Operator's ManualThis Manual includes instructions for using Pearl Imager Accessories including the Pearl Docking Station and Pearl Clean Box.Manual
Pearl Trilogy ManualOperator's Manual for the hardware specifications and operations of the Pearl Trilogy Imager.Manual
Moving Instructions for the Pearl Trilogy Small Animal ImagerInstructions for moving the Pearl Trilogy Small Animal Imager within the lab.Quick Start
Quick Start Guide for the Pearl Trilogy Imager with Image Studio Software 5.0This Quick Start Guide introduces using the Pearl Trilogy Imager with Image Studio 5.0, including Work Area creation, image acquisition and display, data analysis, exporting images, and exporting data.Quick Start
Compound Injection Clip Pack Insert (P/Ns 9300-850, 9300-851)The compound injection clip can be used with the Pearl Impulse Imaging System for in vivo imaging.Accessory Insert
Pearl Imaging Bed Pack Insert (P/Ns 9957-023 and 9300-021)The Pearl Imaging Bed is an accessory for the Pearl Impulse Small Animal Imaging System.Pack Insert
Organ Tray Base Pack Insert (P/N 9300-23)Pack insert for the Organ Tray Base accessory that can be used on the Pearl instrument for in vivo imaging.Accessory Insert
Organ Imaging Tray: Pack Insert for p/n 9300-836Organ Imaging Tray: Pack Insert for p/n 9300-836.Accessory Insert
Pearl Imager CartThis document has instructions for the Pearl Imager Cart.Accessory Insert
Pearl_Source_Licenses.pdfPearl Source LicensesLicense Information
SmartFlow Anesthesia Suite Quick Start Guide for p/n 9000-101 and 9000-102Guide for using the Anesthesia Suite to supply Isoflurane anesthesia gas to the Pearl Impulse, Pearl Imager, MousePOD Accessory, or Pearl Docking Station.Quick Start
SmartFlow Anesthesia Suite Quick CardUser guide for the general operation of the SmartFlow Anesthesia Suite.Quick Card
Image Studio Connection Guide (Odyssey Family, Pearl, and Aerius Imagers)This Guide explains how to connect the Odyssey Classic, Odyssey CLx, Odyssey Fc, C-DiGit Blot Scanner, Pearl Imager, and Aerius Imager to Image Studio Software.Quick Start
Locating Your LI-COR Instrument Serial NumberThis Guide explains how to find the serial number on any Odyssey Imaging System, Pearl Imager, or 4300 DNA Analyzer.Quick Start
Western Blot Documentation WorksheetUse this worksheet to keep track of experimental details. Good record-keeping is essential for performing reproducible Western blots.Quick Card
Empiria Studio Software Sample Images GuideThis guide provides the data needed to analyze the Sample Images available at licor.com/empiria-support.Application Guide
EULA-EmpiriaStudio-v1.3.pdfEmpiria Studio End User's License AgreementEULA
ESLicense.pdfEmpiria Studio Version 2.3 End User's License AgreementEULA
LASLicense.pdfImage Studio™ Software Version 1.2 End User's License AgreementEULA
End-User License Agreement for Image Studio 2End-user license agreement for Image Studio V2 software.EULA
Image Studio Software Version 2.0 Help SystemHelp system for Image Studio Software version 2.0.Help System
End-User License Agreement for Image Studio 3End-user license agreement for Image Studio V3 software.EULA
Image Studio 4 End-User License AgreementEnd-user license agreement for Image Studio V4 software.EULA
Image Studio 5 End-User License AgreementEnd-user license agreement for Image Studio V5 software.EULA
image_studio2_licenses.pdfImage Studio 2 license informationLicense Information
image_studio3_licenses.pdfImage Studio 3 License InformationLicense Information
image_studio4_licenses.pdfImage Studio 4 License InformationLicense Information
Using the Image Studio Connection Diagnostics Utility Program with the Odyssey CLx, Odyssey Fc, C-DiGit Blot Scanner, or Pearl ImpulseThis User Guide explains how to use the Image Studio Connections diagnostic utility.Installation Guide
Quick Start Guide for Image Studio Lite Software 5.xThis Quick Start Guide introduces using Image Studio Lite 5.x, including Work Area creation, image import, image display, data analysis, exporting images, and exporting data.Quick Start Guide
LI‑COR Acquisition Software End User's License AgreementLI‑COR Acquisition Software End User's License Agreement (1.7.2021)EULA
LI‑COR Acquisition Software End User's License AgreementLI‑COR Acquisition Software End User's License Agreement (01.2022)EULA
LI‑COR Acquisition Software End User's License AgreementLI‑COR Acquisition Software End User's License Agreement (11.2021)EULA
Pearl Cam License InformationPearl Cam License InformationLicense Information
In-Cell Western Protocol: Assessing Response of A431 Cells to Stimulation with Epidermal Growth FactorIn-Cell Western protocol for the quantitative determination of EGF receptor and ERK phosphorylation in A431 cells.Example Experiment
Characterization and Performance of a Near-Infraed 2-Deoxyglucose Optical Imaging Agent for Mouse Cancer ModelsMalignant neoplasms exhibit an elevated rate of glycolysis over normal cells. This characteristic can be exploited for optical imaging of tumors in mice. A near infrared fluorophore, IRDye 800CW, emission maximum 794 nm, was conjugated to 2-deoxyglucose (2-DG). An immunofluorescent cell-based assay was used to evaluate specificity and sensitivity of the conjugate in cultured cell monolayers.Application Guide
IRDye 800CW Maleimide Labeling Application GuideIRDye 800CW Maleimide is a functional derivative of infrared dye IRDye 800CW that is reactive toward free–SH (thiol, sulfhydryl) groups. Most molecules that contain free–SH groups can be labeled with maleimide dyes, including IRDye 800CW Maleimide Infrared Dye. This application guide provides guidelines for labeling with IRDye 800CW Maleimide.Application Guide
Near-infrared-labeled tetracycline derivative is an effective marker of bone deposition in miceThis paper explores the use of IRDye 800CW labeled compounds to study bones. By conjugating target compounds to IRDye® 800CW, we extended the effective fluorescence signal detection to the NIR region without affecting the compound’s ability to function as a marker of the mineralization process. Application Guide
Using the Z'-Factor Coefficient to Monitor Quality of Near-Infrared Fluorescent Cell-Based AssaysMany factors affect the quality and reproducibility of microplate-based immunocytochemical assays like the In-Cell Western™ assay. Consequently, an efficient, reliable performance measurement for detection of changes in assay quality is desirable. The Z’-factor coefficient is a simple statistical parameter that is commonly used to assess the quality of high throughput screening (HTS) assays.Application Guide
A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models This review outlines some of the general principles that are important when developing and using fluorochrome-labeled optical contrast agents for oncology investigations in animals.Application Guide
LI‑COR Acquisition Software End User's License AgreementLI‑COR Acquisition Software End User's License Agreement (Rev 11.2021)EULA
PearlCam_License.pdfPearl Cam License InformationLicense Information
In-Cell Western Protocol: Assessing Response of A431 Cells to Stimulation with Epidermal Growth FactorIn-Cell Western protocol for the quantitative determination of EGF receptor and ERK phosphorylation in A431 cells.Example Experiment
Characterization and Performance of a Near-Infraed 2-Deoxyglucose Optical Imaging Agent for Mouse Cancer ModelsMalignant neoplasms exhibit an elevated rate of glycolysis over normal cells. This characteristic can be exploited for optical imaging of tumors in mice. A near infrared fluorophore, IRDye 800CW, emission maximum 794 nm, was conjugated to 2-deoxyglucose (2-DG). An immunofluorescent cell-based assay was used to evaluate specificity and sensitivity of the conjugate in cultured cell monolayers.Application Guide
IRDye 800CW Maleimide Labeling Application GuideIRDye 800CW Maleimide is a functional derivative of infrared dye IRDye 800CW that is reactive toward free–SH (thiol, sulfhydryl) groups. Most molecules that contain free–SH groups can be labeled with maleimide dyes, including IRDye 800CW Maleimide Infrared Dye. This application guide provides guidelines for labeling with IRDye 800CW Maleimide.Application Guide
Near-infrared-labeled tetracycline derivative is an effective marker of bone deposition in miceThis poaper explores the use of IRDye 800CW labeled compounds to study bones. By conjugating target compounds to IRDye® 800CW, we extended the effective fluorescence signal detection to the NIR region without affecting the compound’s ability to function as a marker of the mineralization process. Application Guide
Using the Z'-Factor Coefficient to Monitor Quality of Near-Infrared Fluorescent Cell-Based AssaysMany factors affect the quality and reproducibility of microplate-based immunocytochemical assays like the In-Cell Western™ assay. Consquently, an efficient, reliable performance measurement for de-tection of changes in assay quality is desirable. The Z’-factor coefficient is a simple statistical parameter that is commonly used to assess the quality of high throughput screening (HTS) assays.Application Guide
A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models This review outlines some of the general principles that are important when developing and using fluorochrome-labeled optical contrast agents for oncology investigations in animals.Application Guide
Cell Viability Assay with Sapphire700 Stain and the Odyssey CLx and Sa Imaging SystemsThis protocol describes a cell viability assay that uses near-infrared fluorescent detection. Sapphire700 Stain is used to determine cell viability by assessing cell membrane integrity, and the assay is imaged with the Odyssey CLx and Odyssey Sa Imaging Systems.Application Guide
A High Throughput Drug Discovery for Intracellular Neurodegenerative Disease-Associated Targets using the LI-COR Odyssey Near Infrared SystemThis paper by Chad Dickey discusses a high-throughput method for the quantitative detection of an intracellular target (Tau) in a cell-based assay, the In-Cell Western assay.Application Guide
Electrophoretic Mobility Shift Assay (EMSA) Using IRDye OligonucleotidesThe Odyssey Family of Imagers offers a quick and easily-adapted alternative method to radioisotopic and chemiluminescent detection methods for EMSA analysis and visualization. Application Guide
Glycoprotein Detection with the Odyssey Infrared Imaging SystemApplication Guide
In-Gel Immunochemical Detection of Proteins that Transfer Poorly to MembranesWhen fluorescent secondary antibodies are detected with an Odyssey Imager, nanogram quantities of protein can be specifically detected by the in-gel protocol. A linear fluorescence response is seen over three orders of magnitude in protein concentration.Application Guide
On-Cell Western Plate-Based Assay for Targeted Near-Infrared-Labeled Optical Imaging Agent Development: Receptor-Based Binding and Competition AssaysThis protocol is intended to illustrate the process for testing a particular cell type with the IRDye 800CW EGF for eventual use in vivo. The On-Cell Western Assay is used.Application Guide
One-Blot Western Optimization using the MPX Blotting SystemThe independent channels of the MPX (Multiplex) Blotter make it possible to optimize blocking buffer, primary antibody dilution, and secondary antibody dilution on a single Western blot.Application Guide
In Vivo Animal Imaging Diet ConsiderationsAs presented in this application note, a simple consideration of what diet to feed mice prior to imaging could improve background levels significantly in the abdominal region.Application Guide
Pearl Imager Precision in Organ AnalysisThe Pearl Imager provides uniform area illumination by laser excitation, effectively minimizing variability when imaging animals and organs. This document will illustrate some of these effects and provide guidance when imaging high and low intensity targets (i.e., organs, tissues) in the same field of view. Application Guide
Labeling of Fixed Cells with IRDye NHS Ester Reactive Dyes for In-Cell Western Assay NormalizationA key feature in the In-Cell Western Assay is its ability to simultaneously measure two targets of interest or normalize the data for well-to-well variation in cell number. LI-COR has developed three types of protocols for normalization.Application Guide
Single-Dose Intravenous Toxicity Study of IRDye 800CW in Sprague-Dawley RatsBased on hematologic, clinical chemistry, and histopathologic evaluation, single administration of IRDye 800CW carboxylate intravenously at dose levels of 1, 5, and 20 mg/kg or 20 mg/kg intradermally produced no pathological evidence of toxicity.Application Guide
Quantitative, Two-Color Western Blot Detection with Infrared FluorescenceIn this paper, LI-COR scientists compared the linear range of IR detection to chemiluminescence in a dot blot assay, and found that IR fluorescence provided a 16-250 fold wider quantifiable linear range with increased accuracy.Application Guide
Immunofluorescent Mapping of Cannabinoid CB1 and Dopamine D2 Receptors in the Mouse BrainThis paper demonstrates the utility of infrared immunostaining for rapid and detailed evaluation of expression patterns for two G protein coupled receptors, the cannabinoid CB1 and dopamine D2 receptor in mouse brain.Application Guide
A Cell Based Immunocytochemical Assay For Monitoring Kinase Signaling Pathways and Drug EfficacyWe have developed a near-infrared (NIR) cytoblot assay that can be used to investigate both kinase signaling and the effects of kinase inhibitors.Application Guide
Tracking G Protein-Coupled Receptor Trafficking using Odyssey ImagingThe Miller lab developed a novel way of quantifying the trafficking of CB1 to and from the cell membrane using IR fluorescence. With this technology, the detailed molecular mechanisms involved with GPCR trafficking can be elucidated.Application Guide
The LI-COR Odyssey as a Near-Infrared Imaging Platform for Animal Models of Alzhemier's DiseaseIn this study, the LI-COR Odyssey Imager was used as a detection platform to facilitate the development of new near infrared imaging approaches. The results show that the Odyssey Imager is a flexible imaging tool for molecular imaging of NIR fluorophores.Application Guide
Quantitative methods for analysis of protein phosphorylation in drug developmentThis review examines some of the current formats used for quantifying kinase function for drug development.Application Guide
Bacterial Colony Imaging GuideThis document describes how to use an Odyssey M Imager and LI-COR Acquisition Software to acquire an image of bacterial colonies.Application Guide
In-Cell Western Protocol: Phopho-p53 Detection in COS Cells in Response to HydroxyureaIn-Cell Western protocol for the quantitative determination of p53 phosphorylation in COS cells in response to stimulation with hydroxyurea.Example Experiment
Determining the Linear Range for Quantitative Western Blot DetectionThis protocol explains how to choose an appropriate amount of sample to load for QWB analysis.Protocol
Developing a 3-Color Western Blot: What You Need to Know to Get StartedThis guide presents some things to keep in mind to achieve meaningful results when detecting three unique targets in a Western blot.Application Guide
Ex Vivo Imaging Quick Start GuideNear-infrared labeled targeting agents provide the ability to see deeper into tissues to determine which organs retain the probe and how the probe is retained within different organs.Protocol
Near-Infrared (NIR) Western Blot DetectionThis technical note discusses how to perform a near-infrared fluorescent Western blot. It also covers troubleshooting hints and tips.Protocol
Good Multiplexing Gone Bad: Tips to Target Multiple Proteins on One Western BlotThis guide presents nuances encountered when multiplexing more than one target per membrane and offers potential solutions that do not reduce assay accuracy.Application Guide
Good Normalization Gone BadGood Normalization Gone Bad presents examples of normalization that have been adversely affected by common pitfalls and offers potential solutions.Application Guide
Good Westerns Gone Bad: Maximizing Sensitivity on Chemiluminescent Western BlotsThis troubleshooting guide addresses many common problems seen with chemiluminescent Western blots and correct or improve the issue for great results.Application Guide
Good Westerns Gone Bad: Tips to Make Your NIR Western Blot GreatNear-infrared fluorescence detection on the Odyssey Family of Imaging Systems provides a quantitative two-color detection method for Western blots. This document will discuss some factors that may alter the performance of a NIR Western blot.Application Guide
In-Cell Western Protocol: Complete Apoptosis Assay Example Detailing the Seeding, Induction, and Detection of the HeLa Cellular Response to Anisomycin TreatmentIn-Cell Western protocol for the quantitative determination of apoptosis (cleaved caspase-3) in HeLa cells in response to Anisomycin treatment.Example Experiment
In-Cell Western Protocol: Complete Sample Protocol Detailing the Seeding, Stimulation, and Detection of the Hela Cellular Response to Epidermal Growth FactorIn-Cell Western protocol for the quantitative determination ERK phosphorylation in HeLa cells in response to EGF stimulation.Example Experiment
Housekeeping Protein Normalization ProtocolThis protocol describes how to use a housekeeping protein for Western blot normalization and quantitative analysis.Protocol
Housekeeping Protein Validation ProtocolThis protocol explains how to validate an HKP for use as an internal loading control, by demonstrating that HKP expression is stable in the relevant experimental samples.Protocol
In-Cell Western Assay Development HandbookThe In-Cell Western Assay is a quantitative immunofluorescence assay performed in microwell plates that combines the specificity of Western blotting with the replicability and throughput of ELISA. This handbook explains how to develop a reliable In-Cell Western assay.Application Guide
In-Gel Western Detection Using Near-Infrared Fluorescent DetectionProtocol for performing In-Gel Westerns with near infrared fluorescence for hydrophobic or large proteins or glycoproteins. Image with the Odyssey CLx, Odyssey Classic or Odyssey Fc Imaging System.Protocol
IRDye Peptide Labeling Application GuidePeptides labeled with fluorescent dyes are important as probes for in vivo imaging and as substrates for en-zyme activity assays. The IRDye infrared dye NHS esters will react with the amino groups of a peptide to form a stable, specifically-labeled compound. Application Guide
In-Cell Western Protocol: Complete Sample Protocol Detailing the Seeding, Stimulation, and Detection of the NIH3T3 Cellular response to PDGF-BBIn-Cell Western protocol for the quantitative determination of AKT phosphorylation in NIH3T3 cells in response to PDGF-BB stimulation.Example Experiment
Complete Sample Protocol Detailing the Seeding, Stimulation, and Detection of the NIH3T3 Cellular Response to acidic Fibroblast Growth FactorIn-Cell Western assay protocol to assess the effect of acidic FGF on NIH3T3 cells.Example Experiment
Western Blot Normalization HandbookThe Normalization Handbook describes how to choose and validate an appropriate internal loading control for normalization.Application Guide
Imaging Nucleic Acid Gels on the Odyssey Fc ImagerThe Odyssey Fc Imager, with 600 channel capabilities, can image agarose gels stained with popular DNA stains, such as ethidium bromide and SYBR Safe DNA stain, with sub-nanogram sensitivity.Application Guide
Syto 60 Staining of Nucleic Acids in GelsNucleic acids stained with the Syto 60 stain can be detected and quantified on the Odyssey® Infrared and Odyssey Fc Imaging Systems using the 700 nm channel.Application Guide
In Vivo Validation of Optical ProbesBefore beginning a large animal study, it is critical to confirm that your labeled targeting agent is specific to the intended target within the animal. Understanding the biodistribution and clearance of a labeled targeting agent is critical for meaningful image analysis and interpretation.Protocol
Optimizing Chemiluminescent Western BlotsThis application note walks through the chemiluminescent Western blot workflow and how to optimize your results using the Odyssey Fc Dual-Mode Imaging System.Application Guide
In-Cell Western Protocol: Phospho-p38 Detection in HeLa Cells in Response to AnisomycinIn-Cell Western Assay for Phospho-p38 Detection in HeLa Cells in Response to Anisomycin.Example Experiment
Antibody Validation for Western blot: By the User, for the UserIn this article, practical strategies are proposed for the validation of primary antibody specificity, selectivity, and reproducibility using Western blot analysis.Article
A systematic approach to quantitative Western blot analysisAttaining true quantitative data from WB requires that all the players involved in the procedure are quality controlled including the user.Article
In-Cell Western Protocol: Complete Sample Protocol for PMA-Induced ERK Activation in Suspension Cell LinesOdyssey In-Cell Western sample protocol for PMA-induced ERK Activation in Suspension Cell Lines.Example Experiment
Detection of Post-immunoprecipitation Proteins by Western blot using Quick Western Kit - IRDye 680RDImmunoprecipitation (IP) is a valuable tool that utilizes antibody:antigen interactions to isolate a specific target protein from thousands of proteins in a complex solution.Protocol
Probe Design and In Vitro Validation Quick Start GuideThis guide provides an overview of labeling a targeting compound and evaluating the labeled targeting compound in vitro.Protocol
Pan/Phospho Analysis For Western Blot NormalizationThis protocol describes how to use pan-specific antibodies as an internal loading control for normalization.Protocol
Quantitative Western Blot Analysis with Replicate SamplesReplicate measurements are critical for quantitative Western blot analysis.Protocol
Revert 700 Total Protein Stain NormalizationThis protocol describes how to use Revert 700 Total Protein Stain for Western blot normalization and analysis.Protocol
IRDye 680LT Maleimide Labeling Application GuideIRDye 680LT Maleimide is a functional derivative of infrared dye IRDye 680LT that is reactive with free –SH (thiol, sulfhydryl) groups. Most molecules that contain free –SH groups can be labeled with maleimide dyes, including IRDye 680LT Maleimide Infrared Dye. The application guide describes how to label with IRDye 680LT Maleimide.Application Guide
In-Cell Western Assay: FAQs for Suspension CellsInformation on how to successfully use non-adherent cells for In-Cell Western Assays.Application Guide
Western Blot and In-Cell Western Assay Detection Using IRDye Subclass Specific AntibodiesThis Technical Note is a guide to using IRDye Subclass Specific antibodies for Western blotting and In-Cell Western Assay (In-Cell Western) applications.Application Guide
Protein Electrotransfer Methods and the Odyssey Infrared Imaging SystemsThis document describes the specific application of these two transfer methods for detection on the Odyssey Infrared Imagers. Application Guide
2DE Gel Detection on the Odyssey Infrared Imaging SystemGeneral guide to adapting 2DE gels for imaging on the Odyssey Infrared Imaging system.Application Guide
In Vivo Image Analysis Options Using Image Studio SoftwareThis technical note discusses how to perform image analysis of in vivo images from the Pearl Impulse Small Animal Imaging System with Image Studio Software.Application Guide
Handbook of Assays for Developing Targeted TherapeuticsThe aim of this handbook is to describe assays you can use to answer vital questions related to therapeutics research. Creative uses of these assays are included to spark ideas.Handbook
Tissue Section Imaging GuideTissue section imaging and microscopy are effective for granular identification of targeting agent locations within an organ. With appropriate microscopy equipment, you can assess NIR-labeled targeting agent location.Protocol
In-Cell Western Protocol: Complete Sampling Protocol for Measuring IC50 of Inhibitor U0126 in NIH3T3 Responding to aFGF-1In-Cell Western protocol for the IC50 determination of the MEK inhibitor U0126 on NIH3T3 cells in response to aFGF-1.Example Experiment
Detecting Feline Panleukopenia Virus in CrFK Cells Using Virus TitrationIn this example experiment, we present a protocol for detecting Feline Panleukopenia (FPL) in Crandall Reese Feline Kidney (CrFK) cells.Example Experiment
Odyssey Western Blot Blocker Optimization for Near-Infrared (NIR) DetectionThis document describes a method to determine optimal blocking conditions for NIR Western blot detection with the Odyssey family of Imagers.Protocol
Image Studio 21 CFR Part 11 Compliance GuideThis document describes how Image Studio 21 CFR Part 11 can help your organization comply with the Food and Drug Administration's 21 CFR Part 11.Software Guide
Image Studio Software Background Subtraction Reference GuideImage Studio Software Background Subtraction Reference Guide.Software Guide
How to Use the Image Studio Software In-Cell Western AnalysisA brief overview followed by step-by-step instructions for using the In-Cell Western Analysis (with In-Cell Western key).Software Guide
Image Studio Software Work Area Folder Best PracticesThis Software Guide explains best practices for using a Work Area folder in Image Studio Software.Software Guide
Image Studio Software Work Area Folder Best PracticesThis Software Guide explains best practices for using a Work Area folder in Image Studio Software 5.x.Software Guide
Image Studio Software Export GuideThis technical note explains how to export images and data folders from Image Studio Software, and when each method should be used.Software Guide
Exporting Files from Odyssey Application Software for Various UsesThis Software Guide outlines how to export images and files using Odyssey Application Software.Software Guide
Odyssey Application Software Browser Interface: Acquiring Images and Saving for Image Studio Use This technical note explains how to: take a scan using the Odyssey Application Software Browser Interface, save the image to a computer, and import the image into Image Studio Software.Software Guide
Using Chameleon Protein Ladders in Image Studio Software 1.x-3.xThis Software Guide explains how to add protein markers to a Work Area, size bands using protein markers, and export data using Image Studio Software 1.x-3.x.Software Guide
Using Chameleon Protein Ladders in Image Studio Software 4.0This Software Guide explains how to add protein markers to a Work Area, size bands using protein markers, and export data using Image Studio Software 4.0.Software Guide
How to Use the Image Studio Software Small Animal Image AnalysisA brief overview followed by step-by-step instructions for using the Small Animal Image Analysis (with Small Animal Analysis key).Software Guide
Image Studio System Management Tool GuideThis guide describes how to use the System Management Tool for Image Studio 21 CFR Part 11 Software. Software Guide
How to Use the Image Studio Software Western and MPX Western AnalysesA quick overview of how to perform Western and MPX Western analyses in Image Studio Software followed by step-by-step instructions.Software Guide
Microwell Plate Loading GuidesThese blank plate layouts can be placed under a 96-well microwell plate to make it easier to pipette the correct solutions into the correct wells. Label the rows and columns or color in the wells as appropriate for your experiment.Quick Start
Secondary Antibody Quick CardQuick card with information about secondary antibodies in the .1 mg pack size.Quick Card
Secondary Antibody Quick CardQuick card with information about secondary antibodies in the .5 mg pack size.Quick Card
FieldBrite XT(2) Optical System within the Odyssey Fc Imaging SystemBecause FieldBrite™ XT2 technology offers an optimized optical system, data images only need to be captured once, without the need for post-image data manipulation. This white paper discusses this innovative technology.White Paper
Western blot analysis: Comparison of film and the C-DiGit Blot ScannerThis study compares the performance of two signal capture methods: film exposure and the C-DiGit Chemiluminescent Western Blot Scanner.White Paper
Digital Film for Westerns: The C-DiGit Chemiluminescent Western Blot ScannerThe C-DiGit Blot Scanner is engineered to make Western blot experiments simpler and more productive. Digital film documents your chemiluminescent Western blot results and makes darkroom hassles disappear. Read this white paper for more information.White Paper
Imaging Chemiluminescence by ScanningThe C-DiGit Chemiluminescent Western Blot Scanner is a digital replacement for film, combining the sensitivity of film with the convenience and flexibility of a CCD imager, all offered at an affordable price.White Paper
Chemiluminescent Westerns: How film and photochemistry affect experimental resultsThis paper describes the effects of photochemistry on the response of film to both faint and strong signals, image quality, image clarity, and quantification by densitometry. White Paper
Film and CCD Imaging of Western Blots: Exposure time, signal saturation, and linear dynamic rangeChemiluminescence is a dynamic, enzymatic process that introduces variability and error in your Western Blot experiments. It’s difficult to find the “best” exposure, and the need for multiple exposures limits the reproducibility of your results. read this white paper to learn how chemiluminescent detection introduces variability and error and how you can improve the reproducibility of your Western blot data.White Paper
Near-Infrared Fluorescence Imaging: Seeing Beyond the Visible with IRDye Infrared DyesFluorescent dyes are important labeling tools for life science research. This paper discusses near-infrared fluorescence and its uses in a variety of research applications.White Paper
Western Blot Normalization: Challenges and Considerations for Quantitative AnalysisResearchers rely on Western blotting (also called immunoblotting) to detect and compare target proteins in complex samples. This white paper reviews the various normalization strategies that you should consider for your Western blots.White Paper
M13 Forward (-29) IRDye 800 Value PackP/N 4000-20HPack Insert
M13 Forward (-29) IRDye 700 Value PackP/N 4200-20GPack Insert
50-350 BP DNA Sizing StandardP/Ns: 4200-44, 4000-44BPack Insert
50-700 BP Sizing StandardP/Ns: 4200-60, 4000-45Pack Insert
KB(Plus)™ 10X TBE Buffer PackP/N 826-05213Pack Insert
KB(Plus)™ 3.7% Gel MatrixP/N 827-05214Pack Insert
KB(Plus)™ 5.5% Gel MatrixP/N 827-05215Pack Insert
KB(Plus)™ 6.5% Gel MatrixP/N 827-05607Pack Insert
Odyssey Demo Kit IP/N 827-06894Pack Insert
Odyssey Demo Kit IIP/N 827-06895Pack Insert
CellTag 700 Stain In-Cell Western Demo Kits I and II (with Odyssey Blocking Buffer)P/Ns: 827-13640, 827-13641Pack Insert
CellTag 700 Stain Kits I and II Demo PackP/Ns: 827-13640, 827-13641Pack Insert
M13 Forward (-29) IRDye 800 PrimerP/N 829-05565Pack Insert
M13 Reverse IRDye 800 Budget PrimerP/N 829-05566Pack Insert
M13 Forward (-29) IRDye 700 PrimerP/N 829-05570Pack Insert
M13 Reverse IRDye 700 Budget PrimerP/N 829-05571Pack Insert
50-350 BP Concentrated Sizing StandardP/Ns: 829-06157, 829-06158Pack Insert
Odyssey Western Blotting Starter Kit 1 (with OBB)P/N 925-34010Pack Insert
Odyssey Western Blotting Starter Kit 2 (with OBB)P/N 925-34011Pack Insert
Odyssey Western Blotting Starter Kit 3 (with OBB)P/N 925-34014Pack Insert
Odyssey Western Blotting Starter Kit 4 (with OBB)P/N 925-34015Pack Insert
IRDye 680RD EGF Optical ProbeP/N 926-08445Pack Insert
IRDye 800CW/QC-1 CSP-3P/N 926-08590Pack Insert
IRDye 680RD RGD Optical ProbeP/N 926-09888Pack Insert
ROSstar 550 ProbeP/N 926-20000Pack Insert
IRDye 800CW YC-27 Optical ProbeP/N 926-27000Pack Insert
ROSstar 650 ProbeP/N 926-30000Pack Insert
Odyssey Western Blotting Kits LT (PBS)P/Ns: 926-31062, 926-31064, 926-31066, 926-31068Pack Insert
In-Cell Western Assay Kits I and IIP/Ns: 926-31070, 926-31072Pack Insert
Odyssey Western Blotting Kits RD (PBS)P/Ns: 926-31081, 926-31082, 926-31083, 926-31084Pack Insert
Chemi-IR Detection Kit, 20 Western BlotsP/N 926-32234Pack Insert
Chemi-IR Detection Kit, 100 Western BlotsP/N 926-32236Pack Insert
Odyssey Western Blotting Kits RD (TBS)P/Ns: 926-34010, 926-34011, 926-34014, 926-34015Pack Insert
Odyssey Western Blotting Kits LT (TBS)P/Ns: 926-34012, 926-34013, 926-34016, 926-34017Pack Insert
ELISA HRP Substrate (680) PackP/N 926-34300Pack Insert
ELISA AP Substrate (680) PackP/N 926-34301Pack Insert
CellTag 700 Stain In-Cell Western Kits I and II (with OBB PBS)P/Ns: 926-41091, 926-41092Pack Insert
IRDye 680RD HA Optical ProbeP/N 926-50000Pack Insert
IRDye 800CW HA Optical ProbeP/N 926-50001Pack Insert
Quick Western Kit - IRDye 680RD (with OBB PBS)P/N 926-68100Pack Insert
ROSstar 800CW ProbeP/N 926-80000Pack Insert
WesternSure ECL SubstrateP/Ns: 926-80020, 926-80100, 926-80200, 926-80500Pack Insert
WesternSure ULTRA Chemiluminescent SubstrateP/N 926-85000Pack Insert
Blocking Buffer Optimization KitP/N 927-40040Pack Insert
Western Blocking Buffer Sample PackP/N 927-40050Pack Insert
Tris Glycine Buffer, 10X Liquid Concentrate P/N 928-40010Pack Insert
Tris Glycine Dry Powder BufferP/N 928-40012Pack Insert
Tris Glycine/Sodium Dodecyl Sulfate Buffer, 10X Liquid ConcentrateP/N 928-40014Pack Insert
Tris Glycine SDS Dry Powder BufferP/N 928-40016Pack Insert
Phosphate Buffered Saline (PBS), 10X Dry Buffer PacksP/N 928-40020Pack Insert
Casein Blocking BufferP/Ns: 927-40300, 927-40200Pack Insert
IRDye 800 PhosphoramiditeP/N 4000-33Pack Insert
IRDye 800CW Goat anti-Mouse IgG Sample PackP/N 827-08364Pack Insert
IRDye 800CW Goat anti-Rabbit IgG Sample PackP/N 827-08365Pack Insert
IRDye 680LT Goat anti-Mouse IgG Sample PackP/N 827-11080Pack Insert
IRDye 680LT Goat anti-Rabbit IgG Sample PackP/N 827-11081Pack Insert
In-Cell Western™ Assay Demo Kit I Pack Insert827-14642Pack Insert
In-Cell Western™ Assay Demo Kit II Pack Insert827-14643Pack Insert
Odyssey Protein Molecular Weight Marker Sample PackP/N 827-40000Pack Insert
50-1500 bp DNA Sizing StandardP/Ns: 829-05350, 829-05351Pack Insert
50-1500 bp Two-Color DNA Sizng StandardP/N 829-05352Pack Insert
Odyssey EMSA KitP/N 829-07910Pack Insert
IRDye 700 p53 Consensus OligonucleotideP/N 829-07921Pack Insert
IRDye 700 Stat3 Consensus OligonucleotideP/N 829-07922Pack Insert
IRDye 700 CREB Consensus OligonucleotideP/N 829-07923Pack Insert
IRDye 700 NFkB Consensus OligonucleotideP/N 829-07924Pack Insert
IRDye 700 AP-1 Consensus OligonucleotideP/N 829-07925Pack Insert
IRDye 700 Sp-1 Consensus OligonucleotideP/N 829-07926Pack Insert
Blue Stop SolutionP/Ns: 829-04459, 830-05629, 830-05630Pack Insert
IRDye 800CW Donkey anti-Chicken IgG , 0.1 mgP/N 925-32218Pack Insert
IRDye 800CW Streptavidin, 0.1 mgP/N 925-32230Pack Insert
Western Blotting Starter Kit 1 (TBS/PVDF)P/N 925-35010Pack Insert
Western Blotting Starter Kit 2 (TBS/PVDF)P/N 925-35011Pack Insert
Western Blotting Starter Kit 3 (TBS/NITRO)P/N 925-35014Pack Insert
Western Blotting Starter Kit 4 (TBS/NITRO)P/N 925-35015Pack Insert
IRDye 680LT Streptavidin, 0.1 mgP/N 925-68031Pack Insert
IRDye 680RD Streptavidin, 0.1 mgP/N 925-68079Pack Insert
IRDye 800CW EGF Optical ProbeP/N 926-08446Pack Insert
IRDye 800CW 2-DG Optical ProbeP/N 926-08946Pack Insert
IRDye 680RD BoneTag Optical ProbeP/N 926-09374Pack Insert
IRDye 800CW BoneTag Optical ProbeP/N 926-09375Pack Insert
IRDye 800CW RGD Optical ProbeP/N 926-09889Pack Insert
Revert 700 Total Protein Stain ProductsP/Ns: 926-11010, 926-11011, 926-11012, 926-11013, 926-11014, 926-11015, 926-11016Pack Insert
Odyssey Loading IndicatorsP/Ns: 926-20002, 926-20004, 926-20012, 926-20014Pack Insert
Western Blotting Kits RD with PBS Blocking BufferP/Ns: 926-32081, 926-32082, 926-32083, 926-32084Pack Insert
Western Blotting Kits RD with TBS Blocking BufferP/Ns: 926-35010, 926-35011, 926-35014, 926-35015Pack Insert
CellTag™ 700 StainP/N 926-41090Pack Insert
CellTag™ 700 Stain Kits I and IIP/Ns: 926-42091, 926-42092Pack Insert
β-Actin Rabbit Monoclonal AntibodyP/N 926-42210Pack Insert
β-Tubulin Rabbit Polyclonal AntibodyP/N 926-42211Pack Insert
β-Actin Mouse Monoclonal AntibodyP/N 926-42212Pack Insert
α-Tubulin Mouse Monoclonal AntibodyP/N 926-42213Pack Insert
COX IV Rabbit Monoclonal AntibodyP/N 926-42214Pack Insert
VRDye™ 490 Goat anti-Mouse IgG, 0.5 mgP/N 926-49010Pack Insert
VRDye™ 490 Goat anti-Rabbit IgG, 0.5 mgP/N 926-49020Pack Insert
VRDye™ 490 Goat anti-Mouse IgG Sample Pack, 25 uLP/N 926-49110Pack Insert
VRDye™ 490 Goat anti-Rabbit IgG Sample Pack, 25 uLP/N 926-49120Pack Insert
IRDye 800CW PEG Contrast AgentP/N 926-50401Pack Insert
VRDye™ 549 Goat anti-Mouse IgG. 0.5 mgP/N 926-54010Pack Insert
VRDye™ 549 Goat anti-Rabbit IgG. 0.5 mgP/N 926-54020Pack Insert
VRDye™ 549 Goat anti-Mouse IgG Sample Pack, 25 uLP/N 926-54110Pack Insert
VRDye™ 549 Goat anti-Rabbit IgG Sample Pack, 25 uLP/N 926-54120Pack Insert
IRDye 680LT Streptavidin, 0.5 mgP/N 926-68031Pack Insert
Quick Western KitP/N 926-69100Pack Insert
10X Orange Loading DyeP/N 927-10100Pack Insert
Intercept Blocking BuffersP/Ns: 927-60000,927-60001,927-60003,927-60010,927-60025,927-60050,927-70000,927-70001,927-70003,927-70010,927-70025,927-70050Pack Insert
927-750xx-650xx_intercept-diluent.pdf927-65001,927-65003,927-65010,927-75001,927-75003,927-75010Pack Insert
Intercept T20 Protein-Free Antibody DiluentsP/Ns: 927-85001,927-85003,927-85010,927-95001,927-95003,927-95010Pack Insert
IRDye 800CW Protein Labeling Kit - High MWP/N 928-38040Pack Insert
IRDye 800CW Protein Labeling Kit - Low MWP/N 928-38042Pack Insert
IRDye 800CW Protein Labeling Kit - MicroscaleP/N 928-38044Pack Insert
IRDye 680LT Protein Labeling Kit - High MWP/N 928-38066Pack Insert
IRDye 680LT Protein Labeling Kit - Low MWP/N 928-38068Pack Insert
IRDye 680LT Protein Labeling Kit - MicroscaleP/N 928-38070Pack Insert
IRDye 680RD Protein Labeling Kit - High MWP/N 928-38072Pack Insert
IRDye 680RD Protein Labeling Kit - Low MWP/N 928-38074Pack Insert
IRDye 680RD Protein Labeling Kit - MicroscaleP/N 928-38076Pack Insert
4X Protein Sample Loading BufferP/N 928-40004Pack Insert
Sapphire700 StainP/N 928-40022Pack Insert
VRDye Protein Labeling Kit - High MWP/Ns: 928-49040, 928-54040, 928-65040Pack Insert
VRDye Protein Labeling Kits - High MW-MicroscaleP/Ns: 928-65044, 928-54044, 928-49044Pack Insert
P/Ns: 929-60011, 929-65011Pack Insert
CellTag 520 Stain Kits III and IV Pack InsertP/Ns: 926-42093, 926-42094Pack Insert
CellTag 520 Stain Pack InsertP/Ns: 926-41094Pack Insert
Chameleon 700 Pre-stained Protein LadderP/N 928-70000Pack Insert
Chameleon 800 Pre-stained Protein LadderP/N 928-80000Pack Insert
Chameleon Duo Pre-stained Protein Ladder P/N 928-60000Pack Insert
Chameleon Kit Pre-stained Protein LadderP/N 928-90000Pack Insert
Chameleon Vue Pre-stained Protein LadderP/N 928-50000Pack Insert
GAPDH Rabbit Monoclonal Antibody, 100 uL926-42216Pack Insert
Histone H3 Mouse Monoclonal Antibody, 100 uL926-42218Pack Insert
Histone H3 Rabbit Monoclonal Antibody, 100 uL926-42219Pack Insert
Intercept Blocking Buffer and Antibody Diluent KitsP/Ns: 927-66003,927-76003Pack Insert
Intercept Protein-Free Blocking BuffersP/Ns: 927-80000,927-80001,927-80003,927-80010,927-90000,927-90001,927-90003,927-90010Pack Insert
IRDye 680RD Protein Labeling Kit - High MW Quick Card928-38072Quick Card
IRDye 680RD Protein Labeling Kit - Low MW Quick Card928-38074Quick Card
IRDye 680RD Protein Labeling Kit - Microscale Quick Card928-38076Quick Card
IRDye 680RD Streptavidin, 0.5 mgP/N 926-68079Pack Insert
IRDye 700 Phosphoramidite4200-33Pack Insert
IRDye 800CW Donkey anti-Chicken IgG, 0.5 mgP/N 926-32218Pack Insert
irdye-800cw-donkey-anti-goat-igg.pdf926-32214Pack Insert
IRDye 800CW Donkey anti-Guinea Pig IgG, 0.5 mgP/N 926-32411Pack Insert
IRDye 800CW Donkey anti-Mouse IgG, 0.5 mgP/N 926-32212Pack Insert
IRDye 800CW Donkey anti-Rabbit IgG, 0.5 mgP/N 926-32213Pack Insert
IRDye 800CW Goat anti-Human IgG, 0.5 mgP/N 926-32232Pack Insert
IRDye 800CW Goat anti-Mouse IgG, 0.5 mgP/N 926-32210Pack Insert
IRDye 800CW Goat anti-Mouse IgG1, 0.5 mgP/N 926-32350Pack Insert
IRDye 800CW Goat anti-Mouse IgG2a, 0.5 mgP/N 926-32351Pack Insert
IRDye 800CW Goat anti-Mouse IgG2b, 0.5 mgP/N 926-32352Pack Insert
IRDye 800CW Goat anti-Mouse IgGM (mu chain specific) 0.5 mgP/N 926-32280Pack Insert
IRDye 800CW Goat anti-Rabbit IgG, 0.5 mgP/N 926-32211Pack Insert
IRDye 800CW Goat anti-Rat IgG, 0.5 mgP/N 926-32219Pack Insert
IRDye 800CW Protein Labeling Kit - High MW Quick Card928-38040Quick Card
IRDye 800CW Protein Labeling Kit - Low MW Quick Card928-38042Quick Card
IRDye 800CW Protein Labeling Kit - Microscale Quick Card928-38044Quick Card
IRDye 800CW Rabbit Anti-HRPIRDye 800CW Rabbit Anti-HRPPack Insert
IRDye 800CW Streptavidin, 0.5 mgP/N 926-32230Pack Insert
NewBlot™ IR Stripping Buffer Pack InsertP/N 928-40028Pack Insert
NewBlot™ PVDF Stripping Buffer, 5XP/N 928-40032Pack Insert
NewBlot™ Nitro Stripping Buffer, 5XP/N 928-40030Pack Insert
Odyssey Blocking Buffer (PBS)P/Ns: 927-40100, 927-40000, 927-40003, 927-40010Pack Insert
Odyssey Blocking Buffer (TBS)P/Ns: 927-50100, 927-50000, 927-50003, 927-50010Pack Insert
Revert 520 Total Protein Stain Pack InsertP/Ns: 926-10010, 926-10016, 926-10015, 926-10011, 926-10021Pack Insert
Vinculin Rabbit Monoclonal Antibody, 100 uL926-42215Pack Insert
VRDye™ 490 Donkey anti-Rabbit IgG Secondary Antibody Pack InsertP/Ns: 926-49014Pack Insert
VRDye™ 490 Donkey anti-Rabbit IgG Secondary Antibody Pack InsertP/Ns: 926-49013Pack Insert
VRDye™ 490 Streptavidin Pack InsertP/Ns: 926-49030Pack Insert
VRDye™ 549 Donkey anti-Goat IgG Secondary Antibody Pack InsertP/Ns: 926-54014Pack Insert
VRDye™ 549 Donkey anti-Rabbit IgG Secondary Antibody Pack InsertP/Ns: 926-54012Pack Insert
VRDye™ 549 Donkey anti-Rabbit IgG Secondary Antibody Pack InsertP/Ns: 926-54013Pack Insert
VRDye™ 549 Streptavidin Pack InsertP/Ns: 926-54030Pack Insert
VRDye™ 490 Donkey anti-Mouse IgG Secondary Antibody Pack InsertP/Ns: 926-49012Pack Insert
WesternSure PenP/N 926-91000Quick Card
Western Blot Storage BagsP/N 929-95100Pack Insert